Live Chat Support Software

How to calculate Tm of a ZNA oligo?

The approximate Tm of ZNA™ can be calculated applying the following equation:

Tm (ZNA™) = Tm (DNA) + 36z/(N-3.2)

z: number of cationic units

N: number of nucleotides


Sequence ATC GAT CG 8 mers Tm(DNA) + ZNA-2 building block Tm(ZNA™) = 24 + 36*2/(12-3.2) =   32