How to calculate Tm of a ZNA oligo?
The approximate Tm of ZNA™ can be calculated applying the following equation:
Tm (ZNA™) = Tm (DNA) + 36z/(N-3.2)
z: number of cationic units
N: number of nucleotides
Sequence ATC GAT CG 8 mers Tm(DNA) + ZNA-2 building block Tm(ZNA™) = 24 + 36*2/(12-3.2) = 32