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what does OD really mean?

Optical density or OD is a method used to estimate how much DNA you have in solution by measuring the absorbance at 260 nm. 1 OD of DNA is approximately 33 µg. For example 1 OD of desalted oligonucleotide will contain full length product plus failure sequences; where as HPLC or PAGE purified oligonucleotide will contain majority of the full length product. Upon shipping, we provide a Certificate of Analysis which contain information about the molecular weight, TM, ug and nmole of the product.

Visit our oligo online calculator.