What methods do you recommend to conjugate a peptide to a carrier molecule?
The most important factor is that the site of conjugation be not an internal amino acid and that the site of conjugation be specific for a single amino acid. Internal amino acids and multiple conjugation sites will not present the peptide in a way that is most similar to the natural antigen. After that the choice of chemistry will be dependent on the type of amino acids present in the sequence.
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10/22/2007