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How should I resuspend purified RNA?

Resuspend or dissolve the RNA after isolation or purification in a buffer suitable for down-stream experiments.

Choose a suitable buffer that minimizes the hydrolysis of RNA. Sodium citrate buffer is an excellent choice for this purpose.

The chelating agent sodium citrate together with a low pH minimizes hydrolysis of RNA.

Use 1.0  mM sodium citrate at pH 6.5.

Alternatively, you can also use 0.1 mM EDTA, or TE buffer, or ultrapure water, however, a citrate buffer it the better choise.

All buffer solutions need to be free of nucleases.

For storage in solution, dissolve the RNA pellet in nuclease-free buffer and store at -80°C.

Avoid multiple freeze-thaw cycles to minimize degradation.