Bio-Synthesis perform sequencing verification during the gene synthesis process to ensure your gene synthesis product is as intended. Final plasmid constructs are clonally isolated and confirmed by Sanger DNA sequence analysis to at least single-strand depth across the synthesized insert. Purified PCR fragments are sequence verified on both strands, although verification at the fragment ends is limited to single strand depth. Subassemblies and intermediate products may be cloned and verified to contain the correct sequence, as needed. DNA sequence re-verification after scale-up DNA preparation is available as an added option to your gene synthesis order.
On completion of your synthetic gene, Bio-Synthesis delivers an electronic data package in parallel with your construct. The data package includes the original sequencing data files from construct verification to facilitate your independent review.