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How to store dye label probe and what quality control provided by BSI?

Quality Control


All our oligonucleotide undergo vigorous process monitoring and strict quality control. Length and labeling are systematically controlled by PAGE and characterized by MALDI-TOP mass spectrometry analysis. Quantity is determined and validated by UV Absorbance at 260 nm.

You will receive a Quality Assurance Certificate that includes quantitated yield, melting temperature, MW and µg/OD with every oligo.  You will also receive an additional label for use in your records or on an additional vial.

Purification


A wide variety of modifications can be incorporated into an oligonucleotide at the time of synthesis, using aspecialized phosphoramidite chemistries for labeling. However, certain modifications require the use of post-synthetic conjugation strategies in which a primary amine modified oligonucleotide is covalently attached to a dye via NHS ester chemistry. Other cross-linking chemistries are also available depending on particular project specifications. Single HPLC purification is recommended when using phosphoramidite oligo chemistry, otherwise dual HPLC is required for post-synthetic labeling.


Guaranteed Yield


Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo. Please see our yield chart for details.

Delivery times


Expected shipment time for common dye modifiers is 3 - 5 working days.  More exotic modifications are usually completed within 5 - 7 working days. 

Packaging


Lyophilized.


Shipping Conditions


Room temperature.

Storage Conditions


Light sensitive material. Fluorescent dye-labeled oligos are more fragile than unmodified oligos. Their ability to fluoresce will decrease over time. To ensure high quality, store the oligo dry at -20°C in small aliquots. Note also that fluorescent dye-labeled oligos should be stored in the dark as light can slowly degrade the fluorescent moieties.

For optimal long-term storage of fluorescent dye-labeled oligos except Cy5 and Cy3, it is recommended that the oligos be resuspended in a slightly basic solution (i.e., TE at pH 8). If brought to a pH below 7, it has been shown that the oligo can begin to degrade and may be unusable within a few weeks. Cy5 and Cy3 begin to degrade at a pH above 7. For best results, resuspend Cy5 and Cy3 labeled oligos at pH 7, aliquot, lyophilize, and store at -20ºC. Fluorescent dye-labeled oligos can be used up to 6 months from purchase when stored at -20°C in the dark.