What is protein precipitation

Protein precipitation methods are used for the concentration of diluted proteins in solution. The goal is to purify and concentrate contaminated proteins or proteins contained in various matrices such as buffers, detergents or in natural sources, such as blood, urine or other biofluids. Protein precipitation methods are routinely used to concentrate and eliminate interfering molecules prior to electrophoresis or quantitative protein characterization or determinations. The mechanism of protein precipitation is to alter the solvation potential of the solvent. The solubility of the solute is lowered by adding a specific reagent to manipulate the repulsive electrostatic forces between proteins to favor their aggregation.