Enhanced Diagnostic Tools
This tutorial details how a TaqMan® MGB Probe can be designed over a specific region of a template sequence such as an exon-exon junction (intron splice-site). Genomic DNA is often co-extracted with RNA and can therefore serve as a template in downstream processes such as PCR. Designing a TaqMan® MGB Probe over an exon-exon junction should enable the exclusion of genomic DNA as a template in a real-time PCR reaction. This tutorial assumes basic working knowledge of the Primer Express® v2.0 Software. If you are unfamiliar with the software, please first review the following documents.
Note: The documents above can be found electronically on your hard drive in Program Files/Applied Biosystems/Primer Express
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