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Spectra of available Fluorescent Dyes

 
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01/09/2024

Spectra of available Fluorescent Dyes

Spectra of available Fluorescent Dyes
Fluorescent Dye Abs Em Labelling Position Purification Option Alternative to
5' Int 3' HPSF HPLC PAGE
Alexa Fluor 350 [Alexa350] 346 442 x x x   x   AMCA, DyLight350, iFluor350, CF350,
ATTO 390 390 476 x x x   x    
Dy-415 [DY415] 418 467 x x x   x   Coumarin (DEAC)
ATTO 425 [ATTO425] 436 484 x x x   x   Alexa Fluor 425
ATTO 465 [ATTO465] 453 508 x x x   x   LC 480 Cyan 500
Bodipy FL [BOFL] 504 510 x x x   x    
Alexa Fluor 488 [Alexa488] 495 519 x x x   x   CY2 DY-495 ATTO 495
FAM [FAM] 495 520 x     x x x CY2 DY-495 ATTO 495
Fluorescein isothiocyanate [FITC] 495 520 x x x   x   CY2 DY-495 ATTO 495
Fluorescein [FLU] 495 520     x   x   DY-495 ATTO 495
Fluorescein-dT [FLUdT] 494 522   x     x   DY-495 ATTO 495
ATTO 488 [ATTO488] 501 523 x x x   x   Cy2
Oregon Green 488 [OG488] 496 524 x x x   x   Cy2
Oregon Green 514 [OG514] 506 526 x x x   x   Cy2
Rhodamine Green [RGR] 503 528 x x x   x   Cy2
TET [TET] 521 536 x     x x x CAL Fluor Gold 540
ATTO 520 [ATTO520] 516 538 x x x   x   CAL Fluor Gold 540 Rhodamine 6G
JOE [JOE] 520 548 x x x   x   CAL Fluor Gold 540 Rhodamine 6G
Yakima Yellow [YAKYE] 530 549 x       x   VIC CAL Fluor 560
Bodipy 530/550 [BO530] 534 551 x x x   x   VIC CAL Fluor 560
HEX [HEX] 535 556 x     x x x VIC CAL Fluor 560 SIMA
Alexa Fluor 555 [Alexa555] 555 565 x x x   x   Cy3 TRITC & TMR Alexa Fluor 546
Dy-549 [DY549] 553 566 x x x   x   Quasar 570 Dragonfly Orange
Bodipy TMR-X [BOTMRX] 544 570 x x x   x   Quasar 570 Dragonfly Orange
Cyanine3 [CY3] 552 570 x     x x   Quasar 570 Dragonfly Orange TYE 563
ATTO 550 [ATTO550] 554 576 x x x   x   NED Cy3 Rhodamine B & 6G
TAMRA [TAM] 544 576 x   x   x   Rhodamine 6G Rhodamine B
Rhodamine Red [RRE] 560 580 x x x   x   Rhodamine 6G Rhodamine B
ATTO 565 [ATTO565] 563 592 x x x   x   PET Alexa Fluor 594
Fluorescent Dye Abs Em Labelling Position Purification Option Alternative to
5' Int 3' HPSF HPLC PAGE
ROX [ROX] 575 602 x x x   x   Texas Red Alexa Fluor 594
Texas Red [TxRed] 583 603 x x x   x   ROX™ Alexa Fluor 594
Cyanine3.5 [CY35] 588 604 x       x   Alexa Fluor 594 CAL Fluor Red 610
LightCycler 610 [LC610] 590 610 x x x   x   Alexa Fluor 594 CAL Fluor Red 610
ATTO 594 [ATTO594] 601 627 x x x   x   Alexa Fluor 594 CAL Fluor Red 610
DY-480 XL [DY480] 500 630 x x x   x    
DY-610 [DY610] 610 630 x x x   x   Alexa Fluor 610
ATTO 610 [ATTO610] 615 634 x x x   x   Alexa Fluor 610
LightCycler 640 [LC640] 625 640 x x x   x    
Bodipy 630/650 [BO630] 625 640 x x x   x   Alexa Fluor 633
ATTO 633 [ATTO633] 629 657 x x x   x   LIZ Alexa Fluor 633
Alexa Fluor 647 [Alexa647] 650 665 x x x   x   Cy5 Quasar 670
Bodipy 650/665 [BO650] 650 665 x x x   x   Cy5 Quasar 670
ATTO 647N [ATTO647N] 644 669 x x x   x   Cy5 Quasar 670
Cyanine5 [CY5] 649 670 x     x x   Quasar 670 TYE 655
DY-649 [DY649] 655 674 x x x   x   Quasar 670
Cyanine5.5 [CY55] 675 694 x       x   Alexa Fluor 680 TYE 705
ATTO 680 [ATTO680] 680 700 x x x   x   Alexa Fluor 680 Alexa Fluor 700
DY-682 [DY682] 690 709 x x x   x   IRDye 700 Quasar 705
ATTO 700 [ATTO700] 700 719 x x x   x   IRDye 700 Quasar 705
ATTO 740 [ATTO740] 740 764 x x x   x   Cy7 Alexa Fluor 740
DY-782 [DY782] 782 800 x x x   x   IRDye 800
Abs: Absorption = Peak excitation wavelength [nm]
Em: Emission = Peak emission wavelength [nm]
Ext. Coeff.: Molare extinctions coefficient [M-1cm-1]
MW: Molecular weight [g/mol] of the dye labelled on 3' and 5' end of an oligonucleotide. For internal labelling 279 g/mol for the Amino linker needs to be added.