Enhanced Diagnostic Tools
Figure 1: Reaction of free carboxyl modified oligonucleotides using the water soluble cross-linker 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, EDAC or EDCI) for the bio-conjugation to amino groups on target compounds or molecules that can also be located on solid supports such as microarrays.
The use of this strategy allows the immobilization of carboxyl modified oligonucleotides to solid supports such as micro-array slides and various types of aminoalkylated beads. These type of conjugates can be used to design and synthesize molecules that are cell permeable, can stabilize complexes with other oligonucleotides, have enhanced detectability relative to the respective unmodified compounds, as well as for the design of antisense oligonucleotides, 2’-O-methoxyethyl modified oligonucleotides sometimes also called gapmers, and others.
Bio-synthesis now offers a wide variety of 3’-, 5’- and internally carboxyl modified oligonucleotides as well as their conjugates to carbohydrates, peptides, proteins and antibodies (1).
2. E. Jablonski, E. W.Moomaw, R. H.Tullis and J. L.Ruth Nucleic Acid Res., 1986, 14, 6115-6128.
3. J. D. Kahl and M. M. Greenberg J. Org. Chem., 1999, 64 (2), 507–510
4. A.V. Kachalova, T. S. Zatsepin, E. A. Romanova, D. A. Strelenko, M. J. Gait, T. S. Oretskaya Nucleosides, Nucleotides Nucleic Acids. 2000, 19, 1693-1707
5. T. P. Prakash, A. M. Kawasaki, E. A. Lesnik, S. R. Owens, M. Manoharan Org. Lett. 2003, 5, 403-406.
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