Biomolecule-small molecule labelings

Biomolecule-biomolecule conjugations

Immobilization on solid supports

Service Portfolios

Our bioconjugation service area includes:

• Nucleic acid and oligonucletoide modifications, biolabelings, conjugations
• Antibody modifications and conjugations
• Enzyme modifications and conjugations
• Modifications with synthetic polymers and biopolymer conjugations
• Avidin-Biotin System
• Preparation of liposome conjugations
• Macrosphere, Nano particle (gold-labeled) and any surface immobilization

Our Capabilities

We are not only capable of synthesizing synthetic molecules but also able to provide various bioconjugation systems for specific intended applications. Whether it is for tagging proteins to make them chromogenic or fluorescent, labeling molecules with biospecific ligands for subsequent affinity interactions, or cross linking two or more substances to create uniquely active conjugates, the choices are available in our laboratory. Our chemist will examine each project carefully, and customize a conjugation procedure on the basis of their chemical reactivities and other chemical properties that affect their behavior during the conjugation process. This results in products that meet the specifications of the client.

Characterization

Our sizeable investment in state-of-the-art analytical equipment provides industry-leading tools to develop and monitor our process. Complementary techniques, such as analytical chromatography and electrophoresis, are routinely used to verify that specifications are met. Sometimes, if the biopolymer is not too massive, Mass Spectroscopy can be used to determine its molecular weight. However, unless a crystal structure is available or a single unique functional group was used for conjugation, it may be difficult to know the exact conjugation site and the molar ratio of the reactants. Because of this complexity and difficulty, the effort required assessing the composition and purity of a bioconjugate depends largely on the requirements and rigor of the downstream research.