Oligo Modifications

We offer a personalized solutions to assist client cross link various molecules and compound and solid support attachment. More...

• Biomolecule-small molecule labelings
• Biomolecule-biomolecule conjugations
• Immobilization on solid supports

Use antisense oligonucleotides for your gene silencing experiments. We hybrid designs using various types of nucleotide analogs to increase high affinity for a successful knockdown experiments. More....

Bio-Synthesis offers labeling of oligonucleotide or peptide/protein with redox active compounds for electrochemical studies.

• Dicarboxymethylene Blue
• Monocarboxymethylene Blue
• Ferrocene, Ferrocene-C6
• Anthraquinone, Anthraquinone-C6

Bio-Synthesis offers metal chelator oligo incorporation of 2,2’-Dipicolylamine. This is a versatile metal-coordinating ligand capable of forming complexes with common metal ions including Zn²⁺, Ni²⁺, Cu²⁺, or Ag⁺. A tremendous advantage of dipicolylamine is complete compatibility with standard DNA synthesis, cleavage and purification protocols. Other chelating ligands may require nonstandard conditions or additional protection and deprotection steps. This product was manufactured and developed by Syntrix Biosystems Inc. Patents Pending. For Research Use Only.

Pyrene and perylene are fluorescent polycyclic aromatic hydrocarbons that have the ability to form ‘excited state dimers’ known as excimers. This unstructured, long-wavelength emission arises from the formation of a charge-transfer complex between the excited state and the ground state of two fluorescent molecules. In Pyrene-dU and perylene-dU, the hydrocarbon is attached at the 5 position of deoxyuridine through a triple bond and is electronically coupled to the deoxyuridine base. This electronic coupling of the base and the hydrocarbon makes the fluorescence sensitive to the base pairing of the dU portion of the molecule, allowing the discrimination between perfect and one base mismatched targets.

• Pyrene-dU
• Perylene-dU

Photo-control, the use of ultraviolet or visible light to control a reaction, has a number of advantages over other external stimuli:

• Light does not introduce contaminants into the reaction system
• Excitation wavelength can be controlled through the design of the photo-responsive molecule
• Controling irradiation time and/or local excitation is now straightforward

When a photo-responsive molecule is directly attached to DNA as a receptor, photo-regulation of the bioprocess regulated by that DNA molecule could, in principle, be achieved. Such photo-responsive DNA could also be used as a switch in a DNA-based nano-machine. Professor Hiroyuki Asanuma and his group at the department of Molecular Design and Engineering of the Graduate School of Engineering of the Nagoya University (Japan) have developed an efficient method to achieve this goal. They have attached azobenzene to DNA and made it photo-responsive. Azobenzene is a typical photo-responsive molecule that isomerizes from its planar trans-form to the non-planar cis-form after UV-light irradiation with a wavelength between 300 nm and 400 nm (lmax is around 330 nm). Interestingly, the system reverts from the cis-form to the trans-form after further irradiation with visible light (wavelength over 400 nm). This process is completely reversible, and the azobenzene group does not decompose or induce undesirable side reactions even on repeated trans-cis isomerization. By introducing azobenzenes into DNA through D-threoninol as a linker, Asanuma and co-workers succeeded in achieving photo-regulation of:

• Formation and dissociation of a DNA duplex.
• Transcription by T7-RNA polymerase reaction.