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Amino Oligo Modification

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Amino Oligo Modification
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Amino Modifiers

A primary amino group can be used to attach a variety of modifiers (such as fluorescent dyes) to an oligonucleotide or used to attach an oligonucleotide to a solid surface, for example, a primary amine (NH2) at at the 5’-position is used to functionalize the corresponding terminus of the nucleotide for conjugation with e.g. an activated NHS ester or isothiocyanate fluorescent label. The 3'-NH2 modification allows the prevention of the 3' to 5' exonuclease degradation of oligonucleotides in biological fluids such as cell culture medium. Several spacers arm are available, C3, C6, C12 methylene (CH2) or longer and all of them are hydrophobic. Uni-Link TM Amino Modifier provides a free primary amine attached to the 5'-end of an oligo via a six carbon aliphatic spacer arm. It is functionally interchangeable with Amino Modifier C6

Amino-Modifier dA, Amino-Modifier dC, Amino-Modifier dG and both Amino-Modifier dT products can be added in place of a dA, dC, dG and dT residue, respectively, during oligonucleotide synthesis.

Amino-Modifier C3 Desalted Qty. 5'-Mod Internal 3' -mod
250 nmol 15 OD $60 NA $85
1.0 µmol  35 OD $80 NA $140
Amino-Modifier C6 Desalted Qty. 5'-Mod Internal 3' -mod
250 nmol 15 OD $75 NA $85
1.0 µmol  35 OD $85 NA $140
5'-Amino-Modifier C12 Desalted Qty. 5'-Mod Internal 3' -mod
250 nmol 15 OD $110 NA NA
1.0 µmol  35 OD $150 NA NA
Amino-Modifier C7 Desalted Qty. 5'-Mod Internal 3' -mod
250 nmol 15 OD NA NA $115
1.0 µmol  35 OD NA NA $140
Amino C6 dT / C2 dT Desalted Qty. 5'-Mod Internal 3' -mod
250 nmol 15 OD $150 $150 $55
1.0 µmol  35 OD $225 $225 $95
Amino C6 dC Qty. 5'-Mod Internal 3' -mod
250 nmol 15 OD $150 $150 NA
1.0 µmol  35 OD $225 $225 NA
Amino C6 dA Qty. 5'-Mod Internal 3' -mod
250 nmol 15 OD $150 $150 NA
1.0 µmol  35 OD $225 $225 NA
Amino C6 dG Qty. 5'-Mod Internal 3' -mod
250 nmol 15 OD $150 $150 NA
1.0 µmol  35 OD $225 $225 NA
Amio C6 dU Qty. 5'-Mod Internal 3' -mod
250 nmol 15 OD $150 $150 NA
1.0 µmol  35 OD $225 $225 NA
PC Amino Modifier Qty. 5'-Mod Internal 3' -mod
250 nmol 15 OD $150 NA NA
1.0 µmol  35 OD $190 NA NA

Purification

Desalt or cartridge (RP1) purification is acceptable for most common phosphoramidite modifiers. However, additional purification is strongly recommended for modifiers that require NHS-ester chemistry to conjugate a dye through an amine linker such as Molecular Probes dyes.

Minimum Guarantee Purified Yield

Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo. Please see our yield chart for details.

Quality Controls

Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis. Final yields are determined using UV absorbance at OD260 In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate.

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