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DNA Modifications
DNAatto Fluorophore

New set of excellent fluorescent dyes from ATTO-Tec has added to our broad portfolio of labels for oligonucleotides. These activated fluorescent dyes have become the standard procedure for stable labelling of proteins, nucleic acids, ligands and other biomolecules, which are widely used for applications as fluorescence microscopy, flow cytometry, fluorescence in situ hybridisation (FISH), receptor binding assays, or enzyme assays.

ATTO dyes are a series of fluorescent dyes which provide all the crucial properties required for modern fluorescent technologies.

  • Strong absorption (high extinction coefficient)
  • High fluorescence quantum yield
  • High photostability
  • Good water solubility
  • Low triplet formation
  • Ideally suited for bioanalytical applications

This compound requires amino- or thiol- linker with a 6-Carbon spacer arm or by substituting any Amino Modifier C6 dA, dT, dG, dC for internal labeling, a double HPLC purification is required.

Contact your Account Representative for pricing on ATTO labeled oligonucleotides. For additional information, please Contact us

Price/labeling, purification and synthesis is an additional charge

Dye Groups Scales Label position
5' Int 3'
ATTO390 250 nmol $318 Inquire $318
1.0 µmo Inquire Inquire Inquire
ATTO465 250 nmol $246 Inquire $246
1.0 µmo Inquire Inquire Inquire
ATTO465 250 nmol $246 Inquire $246
1.0 µmo Inquire Inquire Inquire
ATTO488 250 nmol $395 Inquire $395
1.0 µmo Inquire Inquire Inquire
ATTO495 250 nmol $288 Inquire $288
1.0 µmo Inquire Inquire Inquire
ATTO520 250 nmol $260 Inquire $260
1.0 µmo Inquire Inquire Inquire
ATTO532 250 nmol $390 Inquire $390
1.0 µmo Inquire Inquire Inquire
ATTO540 Quencher 250 nmol $450 Inquire $450
1.0 µmo Inquire Inquire Inquire
ATTO550 250 nmol $390 Inquire $390
1.0 µmo Inquire Inquire Inquire
ATTO565 250 nmol $273 Inquire $273
1.0 µmo Inquire Inquire Inquire
ATTO580 Quencher 250 nmol $450 Inquire $450
1.0 µmo Inquire Inquire Inquire
ATTO590 250 nmol $280 Inquire $280
1.0 µmo Inquire Inquire Inquire
ATTO594 250 nmol $390 Inquire $390
1.0 µmo Inquire Inquire Inquire
ATTO610 250 nmol $215 Inquire $215
1.0 µmo Inquire Inquire Inquire
ATTO612 Quencher 250 nmol $450 Inquire $450
1.0 µmo Inquire Inquire Inquire
ATTO620 250 nmol $450 Inquire $450
1.0 µmo Inquire Inquire Inquire
ATTO633 250 nmol $450 Inquire $450
1.0 µmo Inquire Inquire Inquire
ATTO637 250 nmol $450 Inquire $450
1.0 µmo Inquire Inquire Inquire
ATTO647N 250 nmol $450 Inquire $450
1.0 µmo Inquire Inquire Inquire
ATTO655 250 nmol $450 Inquire $450
1.0 µmo Inquire Inquire Inquire
ATTO680 250 nmol $450 Inquire $450
1.0 µmo Inquire Inquire Inquire
ATTO700 250 nmol $450 Inquire $450
1.0 µmo Inquire Inquire Inquire
ATTO720 250 nmol $450 Inquire $450
1.0 µmo Inquire Inquire Inquire
ATTO740 250 nmol $450 Inquire $450
1.0 µmo Inquire Inquire Inquire

Purification

A wide variety of modifications can be incorporated into an oligonucleotide at the time of synthesis, using aspecialized phosphoramidite chemistries for labeling. However, certain modifications require the use of post-synthetic conjugation strategies in which a primary amine modified oligonucleotide is covalently attached to a dye via NHS ester chemistry. Other cross-linking chemistries are also available depending on particular project specifications. Single HPLC purification is recommended when using phosphoramidite oligo chemistry, otherwise dual HPLC is required for post-synthetic labeling.

Minimum Guarantee Purified Yield

Due to the chemistry of many modifications, yield will be approximately 40 - 60 % less than a standard oligo. Please see our yield chart for details.

Quality Controls

Every oligo synthesized is strictly controlled for quality by using either MALDI-TOF mass spectrometry or polyacrylamide gel electrophoresis (PAGE) analysis.  Final yields are determined using UV absorbance at OD260.  In addition, we perform QC methods tailored to specific modifications, such as OD ratio measurement where appropriate. 

For additional information, please Contact us.

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