Carboxy-dT can be used in place of a dT in an internal position of the oligo, or as the 5' terminal base. The carboxy-dT modifier allows direct coupling of an oligo to a molecule or surface with a primary amino by a standard peptide coupling or via the intermediate N-hydroxy-succinimide (NHS) ester. This modifier is added to the oligo on the machine at the time of synthesis.
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-20°C To -70°C Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
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