BSI offers design and synthesis of dual-labled oligos where a fluorescent reporter and a fluorescence quencher are placed on the same oligo. This process utilizes a closed-tube system, which permits rapid and sensitive high-throughput assays. Most dual-labeled fluorescent systems employ fluorescent resonance energy transfer (FRET) principals to control the dark versus bright state. FRET has been widely used in various biological applications. DNA, RNA, LNA, and peptide probes incorporating fluorescent donor/non-fluorescent acceptor combinations have been developed primarily for various detection of proteolysis and nucleic acid hybridization, real time PCR or other methods that use differential hybridization to distinguish polymorphisms..

BSI offers following dual labeled RNA probes: