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BioSynthesis offer
design and synthesis of dual-labled oligos where a fluorescent
reporter and a fluorescence quencher are placed on the
same oligo, enable closed-tube system which permit rapid
and sensitive high-throughput assays. Most of dual-labeled
fluorescent systems employ fluorescent resonance energy
transfer (FRET) principals to control the dark versus
bight state. FRET has been widely used in various
biological applications. DNA, RNA, LNA,
Peptide probes incorporating fluorescent donor/non-fluorescent
acceptor combinations have been developed primarily
for various detection of proteolysis and nucleic acid
hybridization, real time PCR or other methods that use
differential hybridization to distinguish polymorphisms.. |
