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Custom Oligo Bioconjugation & Oligonucleotide Conjugation Services

Custom DNA and RNA oligonucleotide conjugation to drugs, polymers, antibodies, proteins, small molecules, fluorescent dyes, imaging probes and PCR detection probe systems.

Oligo-Drug Conjugates (ODC) Oligo-Antibody Conjugates (AOC) Small Molecules Polymer / PEG Imaging Probes PCR Probes
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Overview Platforms Service Options Linker Chemistry Payloads & Partners Speak to a Scientist
Overview

Custom oligo conjugates for delivery, detection, imaging and therapeutic research

Bio-Synthesis provides custom oligo bioconjugation services for research, diagnostic and therapeutic development workflows. Our team supports conjugation of DNA, RNA, antisense oligos, siRNA, probes, peptides, antibodies, proteins, polymers, small molecules, fluorophores and specialty functional groups.

Oligonucleotide bioconjugation expands the functionality of nucleic acids by linking oligos to molecules that improve targeting, detection, solubility, stability, delivery or assay performance, including oligo-drug conjugates, oligo-macromolecule conjugates and oligo-imaging probes.

Capabilities include custom linker selection, site-specific conjugation strategies, purification optimization, analytical QC and scalable production support for complex oligonucleotide conjugates. Custom feasibility review is available for non-standard conjugation strategies, payloads and linker systems.

Diagram showing custom oligonucleotide bioconjugation to drugs, polymers, proteins, small molecules, imaging probes and PCR probes

Site-Specific Conjugation

Terminal, internal or functional-group directed conjugation strategies can be selected based on oligo format and application.

Multiple Conjugate Classes

Support for drugs, polymers, proteins, antibodies, small molecules, fluorophores and diagnostic probe formats.

Analytical QC Support

Purity, identity, labeling efficiency and conjugation performance can be evaluated using appropriate analytical methods.
Bioconjugation Platforms

Flexible oligo conjugation platforms for complex molecule design

Conjugation workflows can be configured around sequence, scale, terminal modification, internal functional group, linker type, target molecule and downstream application.

ODC

Oligo-Drug Conjugates

Custom oligo-drug conjugation strategies for targeted therapeutic research, delivery studies and development applications.

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Y

Oligo-Macromolecule Conjugates

Conjugation of oligonucleotides with antibodies, proteins, enzymes, peptides and biologically active macromolecules.

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SM

Oligo-Small Molecule Conjugates

Custom oligo-drug conjugation strategies for targeted therapeutic research, delivery studies and development applications.

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PEG

Oligo-Polymer Conjugates

PEGylation and polymer conjugation platforms for solubility, stability, spacing and delivery optimization.

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✦

Oligo-Imaging Probes

Fluorescent and imaging probe development for molecular diagnostics, visualization, tracking and signal detection.

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PCR

PCR Detection Probes

PEGylation and polymer conjugation platforms for solubility, stability, spacing and delivery optimization.

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Service Options

Custom oligo bioconjugation programs can be configured by oligo type, functional handle, conjugation partner, stoichiometry, purification method and documentation level.

Parameter Options
Oligo Types DNA, RNA, 2′-OMe, 2′-F, LNA, PS; single- or double-stranded; aptamers; PNA/PMO on request
Handles (oligo) Amine, Thiol, Azide, Alkyne, DBCO/BCN, Aldehyde, Maleimide, Biotin, Digoxigenin
Partners Antibodies, proteins/enzymes, peptides/CPPs, small molecules, lipids (cholesterol, tocopherol), polymers/PEG, nanoparticles (Au, silica, QDs), surfaces
Stoichiometry Defined DoL (e.g., 1–3 oligos/antibody) or ratio ranges; site-selective strategies when available
Purification RP-HPLC, IEX-HPLC, size-exclusion, ultrafiltration/desalting; PAGE optional
Documentation COA with MS/HPLC/UV; optional activity/ELISA; GLP/cGMP documentation upon request

Linker Chemistry Matrix

Selection of linker chemistry depends on conjugation partner, functional group compatibility, stability requirements, cleavability and downstream application.

Linker / Chemistry Reactive Groups Cleavable Advantages Typical Uses
Maleimide–Thiol Thiol ↔ Maleimide No Fast kinetics, selective coupling Antibody-oligo, peptide conjugates
Disulfide Linkers Thiol ↔ Thiol Yes Reductive intracellular release Drug delivery, cleavable payload systems
SPAAC Click Chemistry Azide ↔ DBCO / BCN No Copper-free bioorthogonal reaction Sensitive biomolecules, live-cell applications
CuAAC Click Chemistry Azide ↔ Alkyne No Efficient, highly versatile chemistry General oligo labeling and conjugation
NHS Ester Coupling Amine ↔ NHS Ester No Simple and widely compatible Dyes, proteins, small molecules
Hydrazone / Oxime Aldehyde ↔ Hydrazide / Aminooxy Conditional Mild aqueous conjugation Protein and glycan conjugation
PEG Linkers Multiple formats available No Improves spacing, solubility and PK Therapeutic oligos, delivery systems
Photocleavable Linkers Specialized reactive handles Yes Triggered release by light exposure Controlled release and imaging studies

Additional custom linker systems and specialty conjugation chemistries are available upon request.

Payloads & Partners

Oligonucleotide conjugation projects can be configured around the partner type, target application, preferred chemistry and downstream analytical requirements.

Partner Type Examples Typical Chemistry Applications
Antibody / Fab IgG, Fab, scFv (customer-supplied or sourced) Maleimide-thiol (reduced hinge or engineered Cys), SPAAC, tetrazine-TCO PLA/PEA, immuno-PCR, spatial proteomics, barcoded IHC
Proteins / Enzymes Streptavidin, enzymes, binding domains NHS-amine, SPAAC, CuAAC, oxime/hydrazone Capture systems, reporters, proximity assays
Peptides / CPPs Arg-rich CPPs, targeting peptides Maleimide-thiol, NHS-amine, click Cell uptake, trafficking, targeting
Lipids Cholesterol, stearyl, tocopherol Amide coupling, click, thiol-maleimide Membrane anchoring, delivery enhancement
Polymers / PEG PEG(n), multi-arm PEGs NHS-amine, maleimide-thiol, click PK tuning, spacing, solubility
Nanoparticles / Surfaces Gold, silica, magnetic beads, sensors Thiol-Au, silane chemistry, NHS-amine, click Diagnostics, enrichment, biosensing
Small Molecules / Haptens Biotin, DIG, fluorophores, dyes NHS-amine, click, oxime/hydrazone Detection, capture, imaging

We offer much more than listed — get in touch for custom payload, linker and partner feasibility review.

Applications

Applications across delivery, diagnostics, imaging and assay development

Molecular Diagnostics

  • qPCR probes
  • Molecular beacons
  • Hybridization probes
  • Multiplex assay probes
  • FISH probes

Therapeutic Research

  • ASO conjugates
  • siRNA delivery systems
  • Targeted oligo therapeutics
  • Oligo-drug conjugates
  • RNA delivery research

Imaging & Detection

  • Fluorescent imaging probes
  • Near-IR labels
  • Reporter conjugates
  • Cell tracking probes
  • Signal detection assays

Targeted Delivery

  • Antibody targeting
  • Peptide targeting
  • Receptor-targeting ligands
  • Polymer delivery systems
  • Nanoparticle compatible designs
Quality & Documentation

Analytical QC for custom oligonucleotide conjugates

Analytical support can be aligned to conjugate type, oligo chemistry, label type, intended use and documentation expectations.

Identity & Purity

  • LC-MS as applicable
  • Analytical HPLC / UPLC
  • PAGE options
  • Purity profiling

Conjugation Assessment

  • Labeling efficiency
  • UV/Vis analysis
  • Fluorescence analysis
  • Conjugate ratio evaluation

Program Support

  • CoA options
  • Scale-up support
  • Custom documentation
  • Stability evaluation on request

FAQ

What analytical testing is available for conjugates?
Analytical characterization may include LC-MS, HPLC or UPLC, PAGE, UV/Vis analysis, fluorescence analysis, purity profiling and conjugation efficiency assessment.
Can Bio-Synthesis support oligo-drug conjugates?
Yes. Oligo-drug conjugates can be designed for targeted therapeutic research, delivery studies and development workflows.
What molecules can be attached to oligonucleotides?
Oligos can be conjugated to drugs, small molecules, peptides, antibodies, proteins, polymers, fluorophores, imaging probes and other functional groups depending on project requirements.
What types of oligonucleotides can be conjugated?
Bio-Synthesis supports conjugation of DNA, RNA, antisense oligos, siRNA, sgRNA, aptamers, PCR probes and specialty modified oligonucleotides.
Can custom fluorophores or targeting ligands be incorporated?
 Yes. Custom dyes, ligands, peptides, antibodies, polymers and specialty functional groups can be incorporated based on project requirements.
Can conjugates be scaled beyond discovery batches?
 Yes. Bio-Synthesis can support exploratory research batches and larger-scale development programs depending on chemistry, purity requirements and documentation needs.
More FAQ'sAll FAQ's
Contact & Quote Request

Ready to discuss your oligo bioconjugation project?

For the fastest review, share your oligo sequence or length, modification sites, target molecule, linker preference, scale, purification, analytical QC needs and intended application.
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Need help before quoting?
Email: info@biosyn.com
Phone: 800-227-0627 (US/CAN)
Phone: +001-972-420-8505 (INT)

Related Services

Explore supporting oligonucleotide conjugation capabilities and application-specific probe services.

ODC Macromolecule Small Molecule Polymer Imaging Probes PCR Probes

Fast Quote Checklist

Include oligo type, sequence length, conjugation site, target molecule, scale, purification, buffer and documentation needs.

Why Choose Bio-Synthesis

Trusted by biotech leaders worldwide for over 45+ years of delivering high quality, fast and scalable synthetic biology solutions.

Greetings Researchers,

Please be advised that any information, guidelines, writeups, and oral/video/graphic content on our website are intended solely as general guidelines.
It is the researcher's sole responsibility to conduct thorough research on the designs of the products intended for their experiments before submitting
their designs to Biosynthesis for review of production feasibility.
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