Enhance oligonucleotide performance with custom spacer & linker modifications - Backed by expert design and full QC from research to GMP-like supply.
Spacer and linker modifications are used when the oligo needs controlled distance from a label, surface, conjugation handle, nanoparticle, protein, lipid, dye or affinity tag. The right spacer can reduce steric hindrance, preserve hybridization, improve solubility, support click chemistry or enable controlled release.
Bio-Synthesis supports short alkyl spacers, extended C9-C12 linkers, flexible hydrophilic Sp18/HEG, PEG and azido-PEG spacers, photocleavable PC spacers, and abasic d/rSpacer designs for structural or enzymatic studies. The live-site product table is preserved below under Spacers & Linkers.
The spacer should be selected based on the intended role: compact distance control, steric relief, hydrophilic mobility, click-ready conjugation, photocleavable release or non-informational abasic spacing.
Use C2-C6 when the design needs short, controlled spacing with minimal added bulk.
Use Sp18/HEG, PEG or longer spacers to reduce steric interference near surfaces, dyes or large payloads.
Azido PEG3/12/24 spacers combine bioorthogonal azide chemistry with tunable PEG distance.
PC spacers support light-triggered release; d/rAbasic sites create programmable non-informational gaps.
Availability by position is indicative. Check marks indicate positions that are commonly supported; ask if a project requires a position not listed.
Preserved live-site product matrix with cleaner formatting for readability.
Use 5′/3′ termini for conjugation; internal placements benefit from flexible PEG/HEG to preserve Tm.
C2-C6 for compact spacing; C12-Sp18/PEG for bulky labels or surfaces.
PC spacers enable reversible capture and on-demand release with UV/visible light, depending on assay conditions.
Azido-PEG spacers support SPAAC/CuAAC; maintain copper-free conditions for sensitive targets.
Flexible hydrophilic spacers such as PEG/HEG generally preserve duplex stability better than short rigid linkers.
Identity by ESI-MS/MALDI and purity by HPLC; optional SEC for high-molecular-weight conjugates.
Control distance between the oligo and dye, peptide, lipid, protein, chelator, surface or nanoparticle.
Provide reach and mobility for chips, beads, membranes, gold nanoparticles and biosensor surfaces.
Reduce quenching, tune hybridization behavior and support controlled capture/release workflows.
Use azido-PEG spacers for selective attachment of dyes, peptides, lipids or other click-ready payloads.
Use PC spacers when your workflow requires reversible capture, light-triggered cleavage or time-resolved release.
Use dSpacer, rSpacer or rAbasic sites for non-informational gaps, repair studies and enzyme probing.
Explore connected modification and conjugation services.
Include sequence, position, spacer/linker type, application, scale, purification and QC needs.
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