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Biotinylated & Affinity-Tagged Oligonucleotides

Custom biotinylated and affinity-tagged DNA/RNA oligos for streptavidin capture, reversible release, hapten detection, immobilization, enrichment and diagnostic assay workflows.

5′ Biotin / Biotin-TEG Internal Bio-dT Dual Biotin Desthiobiotin DIG / DNP Haptens Photocleavable Biotin HPLC / ESI-MS QC
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Overview Selection Guide Product Matrix Applications Design Notes QC FAQ Contact
Overview

Affinity Tags for Capture, Immobilization, Enrichment & Detection

Bio-Synthesis designs and manufactures biotinylated oligonucleotides and affinity-tagged DNA/RNA probes for capture, immobilization, enrichment and detection workflows. Options include 5′ biotin, Biotin-TEG spacers, internal Bio-dT, dual biotin, NHS-biotin, desthiobiotin, DIG/DNP haptens and photocleavable biotin.

These affinity tags support streptavidin/NeutrAvidin capture on magnetic beads, ELONA/ELISA-like plates, microarrays, biosensors, ISH/DIG detection, lateral flow, library preparation and on-bead workflows. Bio-Synthesis supports custom design, synthesis, purification and assay-specific QC from research grade to ISO 9001/13485 production.

Common formats include streptavidin capture oligonucleotides, biotinylated probes, hapten-labeled oligos, desthiobiotin capture-release oligos, and photocleavable biotin oligos for workflows that require immobilization, enrichment, pull-down, detection or controlled release.

Oligo

Biotin / TEG

Strong streptavidin capture with spacer accessibility.

DIG / DNP

Hapten detection with antibody-based signal chains.

Target

Capture

Biotin, dual biotin and Biotin-TEG support strong streptavidin/NeutrAvidin binding on beads, plates and biosensor surfaces.

Release

Desthiobiotin and photocleavable biotin enable reversible or triggered release for enrichment and library workflows.

Detection

DIG and DNP haptens support antibody-based detection where biotin background or multiplexing is a concern.

Biotinylated Oligos

Reversible Capture

Hapten Detection

Photocleavable Release

Product Matrix

QC Support

Affinity Tag Selection Guide

Choose the Affinity Tag by Workflow

Different affinity tags solve different assay problems. Use standard or spacered biotin for strong capture, desthiobiotin for reversible elution, DIG/DNP for antibody-based detection and PC-biotin for light-triggered release.

Bio

Biotinylated Oligos

High-affinity streptavidin/NeutrAvidin capture for beads, chips, microarrays, ELONA, biosensors and lateral-flow workflows.

5′ Biotin Biotin-TEG Bio-dT Dual Biotin

Explore Biotin Products →

Rev

Desthiobiotin Oligos

Reversible streptavidin capture with mild competitive elution using free biotin for capture-release workflows.

Desthiobiotin NHS Desthiobiotin TEG Azide Tag

Explore Reversible Tags →

DIG

DIG / DNP Haptens

Antibody-detectable haptens for ISH, ELISA-like detection, multiplex assays and reduced endogenous biotin background.

DIG NHS DNP-TEG Anti-hapten Detection

Explore Hapten Labels →

PC

Photocleavable Biotin

UV-cleavable affinity tag for releasing oligos from streptavidin on demand in library prep and bead workflows.

PC-Biotin UV Release Capture & Release

Explore PC-Biotin →

Need maximum retention?

Use dual biotin or Biotin-TEG for stronger surface capture and better accessibility.

Need gentle elution?

Use desthiobiotin when you want reversible streptavidin capture and mild release.

Need antibody detection?

Use DIG or DNP haptens when biotin background or multiplexing is a concern.

Product Matrix

Affinity Tag Product Matrix

The live page separates each product family with technical notes. This version keeps that structure, but reorganizes it into cleaner product-family panels so each matrix is easier to read and the notes stay connected to the correct chemistry.

Biotinylated Oligos: 5′, Internal dT & TEG Spacers

Biotinylated oligos provide high-affinity streptavidin/NeutrAvidin binding for immobilization, pull-downs, probe capture and surface-based assays.

PRODUCT
FUNCTION
APPLICATION
CODE

Biotin deoxythymidine dT

Internal biotin label via dT with high-affinity streptavidin/NeutrAvidin binding.

Immobilization on beads/chips; pull-downs; capture of DNA/RNA probes.

[Bio-dT]

Dual Biotin

Two biotins increase avidity and retention under stringent wash or high-flow conditions.

Robust bead capture; lateral-flow test lines; surface tethering.

[Bio-Dual]

Biotin NHS

NHS-ester biotin for post-synthetic coupling to primary amines such as 5′-amine or amino-link oligos.

Flexible labeling of amine-modified oligos/primers; custom assembly.

[Bio-N]

Biotin TEG 5′

5′ biotin with long TEG spacer to reduce steric hindrance and improve binding accessibility.

Microarrays, biosensors, ELONA capture and magnetic beads.

[Bio-TEG-5]

Biotin-5′

Standard 5′ biotin label for strong streptavidin capture.

General immobilization, pull-downs and lateral-flow conjugates.

[Bio-5]

TEG spacer vs. 5′ biotin: TEG is PEG-like and increases accessibility on crowded surfaces; it often improves binding kinetics.

NHS-biotin coupling: React at pH 7.5–8.5 in anhydrous buffer; avoid Tris. Quench and purify by HPLC.

Internal biotin dT: Choose positions away from critical hybridization sites to avoid duplex disruption.

Desthiobiotin Oligos: Reversible Streptavidin Capture

Desthiobiotin supports capture-and-release workflows where reversible streptavidin binding and mild elution are preferred.

PRODUCT
FUNCTION
APPLICATION
CODE

Desthiobiotin NHS

Biotin analog enabling gentle, reversible elution with added biotin.

Affinity purification with mild release; on-bead assays.

[DesBio-N]

Desthiobiotin TEG

Desthiobiotin plus TEG spacer for low-steric, reversible binding.

Capture-and-release workflows; bead or chip cycling.

[DesBio-TEG]

Desthiobiotin TEG Azide

Reversible tag with terminal azide for CuAAC/SPAAC click attachment.

Click-to-surface assembly; modular probe fabrication.

[DesBioTEG-N3]

Elution strategy: Elute desthiobiotin-bound oligos by competing with free biotin; this can help preserve protein or complex integrity.

When to use: Prefer desthiobiotin for capture-release workflows; use dual biotin when maximum retention is required.

DIG/DNP Hapten-Labeled Oligos: Antibody Detection

DIG and DNP haptens provide antibody-detectable alternatives to biotin for ISH, immunodetection, multiplex assays and reduced endogenous biotin background.

PRODUCT
FUNCTION
APPLICATION
CODE

Digoxigenin NHS

Hapten recognized by anti-DIG antibodies; avoids endogenous biotin background.

In situ hybridization, ELISA-like detection and multiplex immunodetection.

[Dig-N]

DNP TEG

2,4-dinitrophenyl hapten detected by anti-DNP antibodies; TEG spacer improves accessibility.

Immuno-PCR, multiplex bead assays and secondary confirmation labels.

[DNP-TEG]

DIG/DNP vs. biotin: Use DIG or DNP when endogenous biotin causes background or when multiplex detection with anti-hapten antibodies is needed.

Signal chains: Anti-DIG or anti-DNP antibodies can be HRP/AP-conjugated for chemiluminescent readout.

Photocleavable Biotin: PC-Biotin Oligos

PC-biotin provides UV-cleavable release from streptavidin systems for bead release, library preparation and single-molecule workflows.

PRODUCT
FUNCTION
APPLICATION
CODE

PC Biotin

UV-cleavable linker releases oligos from streptavidin on demand.

Library prep and bead release; single-molecule workflows; on-bead synthesis.

[PCBio]

Cleavage conditions: Typical cleavage occurs around 350–365 nm; verify intensity and exposure to avoid sample damage.

Sample protection: Minimize UV exposure for sensitive fluorophores or proteins; consider shielding non-target regions.

Applications

Common Affinity-Tagged Oligo Workflows

CAP

Streptavidin Capture

Magnetic beads, NeutrAvidin plates, biosensors and microarray immobilization.

REL

Reversible Release

Desthiobiotin or photocleavable biotin for gentle elution and on-demand release.

ISH

Hapten Detection

DIG and DNP probes for ISH, ELISA-like detection and multiplex immunodetection.

LAT

Lateral Flow

Dual biotin or Biotin-TEG for strong retention at test lines and flow-based capture zones.

LIB

Library Prep

Capture-release workflows for bead cleanup, selection, enrichment and single-molecule assays.

QC

Diagnostic Assays

ELONA, hybridization probes, immuno-PCR and biosensor probe development with QC support.

Technical Design Notes

Design Guidance for Affinity-Tagged Oligos

Spacer, placement and background control

  • TEG vs. standard biotin: TEG increases accessibility on crowded surfaces and often improves binding kinetics.
  • NHS-biotin coupling: React at pH 7.5–8.5 in anhydrous buffer; avoid Tris; quench and purify by HPLC.
  • Internal Bio-dT: Place away from critical hybridization sites to reduce duplex disruption.
  • DIG/DNP choice: Use anti-hapten detection when endogenous biotin or multiplexing is a concern.

Release and detection strategy

  • Use desthiobiotin for reversible capture and mild competitive elution with free biotin.
  • Use dual biotin when maximum retention is required under stringent wash or high-flow conditions.
  • Use PC-biotin for UV-triggered release, typically around 350–365 nm, while minimizing UV damage to sensitive samples.
  • Confirm affinity-tag performance in the intended bead, plate, membrane or biosensor system.
Quality & Documentation

Purification, QC & Scale Support

PUR

Purification

  • HPLC purification
  • PAGE when required
  • Desalting for selected workflows
  • HPLC + SEC if needed
MS

Analytical QC

  • ESI-MS identity confirmation
  • Analytical HPLC
  • Streptavidin-binding tests on request
  • Assay-specific QC planning
ISO

Documentation

  • Research-grade to ISO 9001/13485
  • COA and method documentation
  • GLP/GMP-aligned workflows as scoped
  • Scale-up support
Scientific & Manufacturing Confidence

Built on Decades of Oligonucleotide Manufacturing Experience

Bio-Synthesis supports affinity-tagged oligonucleotide workflows with U.S.-based oligo manufacturing, analytical QC and conjugation expertise from early research through advanced assay development and regulated environments.

45+ Years of Oligonucleotide Expertise

Custom oligo synthesis and modification support across research, diagnostics and advanced molecular workflows.

Lewisville, Texas U.S. Manufacturing

Centralized production and analytical support with technical coordination for domestic and international programs.

ISO 9001:2015 / ISO 13485:2016

Quality systems supporting research, diagnostic and regulated oligonucleotide manufacturing workflows.

HPLC, LC-MS & Assay-Specific QC

Analytical verification including HPLC purification, ESI-MS identity confirmation and application-specific testing.

Research to GLP/GMP-Aligned Support

Flexible development pathways from RUO through advanced analytical and manufacturing support programs.

Conjugation Handle Modifications Cross-Linking Oligos DNA/RNA Probes Analytical QC Services Therapeutic Oligonucleotide Support

Frequently Asked Questions

FAQ

When should I choose Biotin-TEG instead of standard 5′ biotin?
 Biotin-TEG provides a longer spacer that improves accessibility on crowded surfaces, beads, arrays and biosensors. Standard 5′ biotin is suitable for general streptavidin capture when steric hindrance is not a major concern.
When should I use desthiobiotin?
 Desthiobiotin is useful when reversible streptavidin capture is needed. Bound oligos can be released more gently by competition with free biotin.
Why use DIG or DNP instead of biotin?
 DIG and DNP haptens are useful for antibody-based detection, ISH and multiplex assays, especially when endogenous biotin creates background.
What is photocleavable biotin used for?
 PC-biotin enables UV-triggered release from streptavidin systems and is useful for library prep, bead release, single-molecule workflows and on-bead synthesis.
What QC is recommended?
 Typical QC includes HPLC purification and ESI-MS identity confirmation. Streptavidin-binding tests and assay-specific QC can be requested depending on application.
What should I provide for a quote?
 Send sequence, desired affinity tag, spacer/linker preference, backbone, scale, purification requirement, QC needs, timeline and intended capture or detection workflow.
More FAQ'sAll FAQ's
Contact & Quote Request

Need help designing a biotinylated or affinity-tagged oligo?

Share your sequence, affinity tag, spacer/linker preference, backbone, scale, purification target, QC requirements and intended capture or detection workflow. Bio-Synthesis can recommend the best tag format, placement and analytical strategy.
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Need help before quoting?
Email: info@biosyn.com
Phone: 800-227-0627 (US/CAN)
Phone: +001-972-420-8505 (INT)

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Greetings Researchers,

Please be advised that any information, guidelines, writeups, and oral/video/graphic content on our website are intended solely as general guidelines.
It is the researcher's sole responsibility to conduct thorough research on the designs of the products intended for their experiments before submitting
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