Light-controlled oligonucleotides for hybridization control, photocleavage, uncaging, photorelease, MERFISH signal reset, reversible photoswitching and UV photo-crosslinking.
Photoregulation is the ability of an oligonucleotide system to respond to light. Bio-Synthesis provides custom photocleavable oligonucleotides, photocaged nucleotides such as NPOM-dT and DEACM-dG, azobenzene photoswitch constructs, and CNVK photo-crosslinkers for light-controlled hybridization, release, crosslinking and imaging workflows.
These chemistries are used when an oligo must stay inactive until illuminated, release a captured target from a surface, reset signal for MERFISH/smFISH, switch duplex stability reversibly, or lock a duplex through UV-induced crosslinking. Bio-Synthesis supports application-led design, sequence optimization, spacer geometry, label strategy, HPLC/PAGE purification, LC-MS options, documentation and RUO → GLP scale-up.
Tubes • Plates • Kitting
365 • 405 • 450 nm
UPLC/HPLC • LC-MS • Binding
RUO → GLP
The live-site workflow is preserved, but rebuilt as an animated process rail for faster scanning.
Light enables activation or release, followed by imaging or collection, then signal reset for the next round.
Light removes a cage or cleaves a linker to activate function or release a payload.
Image, collect, or analyze the activated system.
Reset the system for another imaging or assay cycle.
Most users start with the workflow question, not the chemistry. Use this guide to choose the right class before reviewing the product table.
Use photocleavable linkers such as PC-Linker, PC-Spacer C3, PC-Biotin or PC-Amino C6.
Use caged nucleotides such as NPOM-dT or DEACM-dG to block pairing until light exposure.
Use azobenzene to toggle duplex stability with light without permanently cleaving or crosslinking.
Use CNVK for UV-induced interstrand crosslinking to trap duplexes or capture interactions.
Use photocleavable readouts for MERFISH/smFISH strip-and-rerun or signal-reset workflows.
Use PC-Biotin or a photolabile handle for streptavidin/surface capture followed by gentle photorelease.
Use caged nucleobases or caged siRNA designs where gene silencing must be activated by light.
Choose 405-friendly DEACM or tune dose, power density and exposure to protect dyes, tissue and cells.
This preserves the live-site product table and reorganizes each chemistry by function, application and code.
Choose by function: photorelease, uncaging/activation, reversible photoswitching or irreversible photo-crosslinking.
Representative photo-regulated and photocleavable oligonucleotide chemistries.
Light-controlled oligo performance depends on wavelength, power density, exposure time, sample sensitivity and the optical setup.
Use these values as design planning ranges; final dose should be validated in the actual instrument and sample type.
Wavelength and exposure planning guide for photo-regulated oligonucleotides.
Design note: Always balance photolysis efficiency with sample safety. Dyes, tissue, cells and hydrogel/surface systems may require lower dose, 405-friendly cages or staged validation.
Photoregulated oligos are used when activation, release, reset, switching or crosslinking must happen at a defined time and place.
Photocleavable readouts for strip-and-rerun imaging and multi-round spatial transcriptomics.
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PC-biotin and photolabile handles for affinity capture followed by gentle on-demand release.
NPOM and DEACM caged bases for time-resolved activation and controlled hybridization.
Azobenzene and related chemistries for reversible light-controlled duplex stability.
CNVK and light-triggered crosslinkers for duplex trapping and interaction capture.
Light-cleavable probe systems for imaging, reset, release and controlled signal removal.
Caged siRNA and light-activated RNA interference research designs.
Photolabile linkers combined with dyes, biotin, surfaces, peptides, proteins or payloads.
Related light-controlled, cleavable and imaging probe services.
Include sequence, photogroup, wavelength, application, labels and QC.
Selected references covering photocaged oligonucleotides, light-controlled RNAi, photocaged morpholinos, photocleavable linkers and optochemical control. These citations provide scientific background and design context.
Note: Photoregulated oligo design should be evaluated around wavelength, sample sensitivity, exposure time, photogroup placement, label compatibility, purification and application-specific validation.
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