Stable-isotope labeled DNA and RNA oligonucleotides for LC-MS standards, kinetic isotope effect studies, NMR tracing, and mechanistic research.
Deuterated base modified oligonucleotides incorporate one or more deuterium (²H/D) substitutions at defined nucleobase positions, such as C8 of purines or 5/6 positions of pyrimidines. These stable-isotope substitutions create predictable mass shifts while generally preserving canonical base-pairing behavior.
Deuterated bases are used for LC-MS quantitation, isotope-dilution internal standards, kinetic isotope effect (KIE) studies, NMR/IR tracing, pharmacokinetic experiments, and development of analytical reference standards.
Bio-Synthesis manufactures custom deuterated DNA and RNA oligonucleotides with defined labeling positions, HPLC/UPLC purification options, LC-MS identity confirmation, optional isotopic enrichment reporting, and fit-for-purpose documentation.
Defined ²H label placed at a selected nucleobase position.
Predictable isotope offset supports LC-MS and analytical readouts.
LC-MS, NMR, IR or isotope-dilution quantitation workflows.
Internal standards, KIE studies, tracing or reference controls.
Deuterium labels are compact, stable isotope modifications that provide analytical value without substantially changing the intended oligonucleotide sequence design.
Known mass offsets support isotope-dilution quantitation, recovery studies and matrix-tolerant analytical workflows.
Defined isotope placement can help probe enzyme mechanisms, transition states and rate-limiting steps.
Deuterium labeling supports molecular tracking and spectral assignment in complex systems.
Stable-isotope labels can distinguish analytes from unlabeled background signals during bioanalytical studies.
Compare supported deuterated base options by label position, function, application and ordering code.
Choose the isotope-labeled base according to the desired mass shift, nucleobase position, assay method and analytical validation requirement.
Representative deuterated base-modified phosphoramidites and custom isotope-labeled oligonucleotide controls.
Availability note: Specific deuterated phosphoramidites, isotopic enrichment, scale and lead time should be confirmed during quote review. Customizations: multiple labeled sites, internal placements, mixed backbones such as 2′-OMe or LNA, PEG/hexa-EG spacers, HPLC/UPLC purification, aliquots, kitting and RUO-to-GMP-like documentation are available on request.
Deuterated base projects should be designed around isotope position, H/D exchange risk, analytical resolution and downstream assay requirements.
Common sites include C8 of purines such as G/A and 5/6 positions on pyrimidines such as U/T/C. The label position should balance synthetic stability and analytical resolution.
Phosphoramidites such as 8-D-dmf-dG can be incorporated through standard DNA synthesis workflows under compatible coupling and deprotection conditions.
HPLC/UPLC purification, ESI-LC-MS identity confirmation, expected mass-shift review and optional isotopic enrichment information support analytical use.
Design tip: For quantitative LC-MS assays, place D-labels away from known fragmentation hot spots or validate fragment maps with a pilot duplex before scaling.
Deuterated oligonucleotides support quantitative, mechanistic and traceability-focused analytical workflows.
Stable-isotope internal standards for method development, recovery testing and isotope-dilution quantitation.
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D-labeled oligos can help distinguish analytes, metabolites or degradation products from unlabeled background.
Mechanistic experiments for polymerase, glycosylase, nuclease or other enzymatic DNA/RNA processing workflows.
Application Use Case
Stable isotope placement can support signal assignment and tracking of labeled oligos in complex systems.
Defined isotope-labeled bases for mechanistic studies involving repair, lesion processing and enzymatic pathways.
Custom reference oligos for assay qualification, release testing support, calibration and analytical method verification.
Related analytical and modified-base oligonucleotide services.
Include sequence, label, position, assay, scale and QC.
Selected references covering stable isotope labeling, kinetic isotope effects, deuterium effects in biomolecular systems, and LC-MS isotope-dilution quantitation. These references provide scientific background rather than product-performance claims.
Note: Reference selection should be aligned with the final assay method, isotope position, instrument platform and quantitative workflow. Bio-Synthesis can help align specific literature protocols to the proposed sequence and QC plan.
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