Custom bDNA amplifier probe systems for QuantiGene, ViewRNA, MERFISH, smFISH, FISH, viral RNA panels, oncology biomarkers, and multiplexed nucleic acid detection workflows.
Bio-Synthesis supports custom branched DNA amplifier probe systems for PCR-free nucleic acid detection workflows. Unlike PCR, branched DNA systems amplify the detection signal instead of the target sequence, helping preserve spatial context while generating strong analytical sensitivity.
bDNA amplifier probes use sequential hybridization layers consisting of capture extenders, label extenders, pre-amplifiers, amplifiers, and labeled probes to build tree-like signal amplification structures around the target molecule.
This strategy can generate hundreds to thousands of detectable reporter molecules per target without enzymatic amplification, making bDNA technology useful for MERFISH, smFISH, ViewRNA, QuantiGene assays, spatial transcriptomics, viral RNA detection, oncology biomarkers, and multiplex imaging workflows.
bDNA systems amplify reporter signal rather than copying the nucleic acid target.
Orthogonal probe architectures can support MERFISH, smFISH, ViewRNA, and other multiplex workflows.
Capture extenders, label extenders, pre-amplifiers, amplifiers, and readout probes can be configured around assay needs.
10²–10³×
Typical signal gain
PCR-Free
No target amplification required
Multiplex
MERFISH and smFISH compatible
QC
HPLC, PAGE, LC-MS options
Branched DNA workflows require careful optimization of probe architecture, hybridization performance, signal amplification efficiency, multiplex compatibility, and background reduction.
A bDNA system builds signal in layers: target capture, extender tiling, pre-amplifier docking, branched amplifier recruitment, and final labeled probe detection.
Amplifier and readout probe strategies for high-sensitivity spatial transcriptomics and imaging workflows.
Layered probe systems for in situ RNA detection in cells, tissues, coverslips, and compatible sample formats.
Plate-based branched DNA signal amplification for gene expression, viral RNA, and biomarker panel workflows.
Probe components can be configured around target tiling, branch factor, signal chemistry, multiplex requirements, and documentation needs.
Bio-Synthesis can align bDNA probe deliverables with your analytical, documentation, and program requirements.
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