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Multi-arm DNA, RNA and modified oligonucleotide architectures engineered for multivalent binding, high-density signaling, multiplex hybridization, capture/enrichment, biosensors and advanced probe design.
Branched oligonucleotides incorporate two or more arms extending from a central scaffold or internal branch point. This enables multiple functional groups, labels, ligands or sequence domains on a single compact molecule.
Bio-Synthesis provides end-to-end custom branched oligo synthesis, including collaborative design, arm layout, branch-point strategy, labeling, conjugation, purification, analytical QC and documentation.
Use branched oligos when a standard linear oligo cannot provide enough binding valency, signal density, or multiplex functionality.
Common designs include 2-arm, 3-arm, 4-arm, and dendrimeric oligonucleotides with optional fluorophores, biotin, reactive handles, PEG spacers, and analytical QC.
Branched oligos consolidate multiple binding, reporting and capture functions into a single engineered nucleic acid construct.
Present identical or orthogonal binding domains to boost avidity, target capture and local concentration effects.
Carry multiple dyes, labels or affinity groups on one scaffold to raise signal without increasing probe count.
Combine distinct sequences on separate arms for simultaneous hybridization events or multi-target detection.
Consolidate capture, reporting, targeting and spacing into one molecule to simplify assay architecture.
Add fluorophores, quenchers, biotin, digoxigenin, reactive handles, PEG spacers or conjugation chemistry.
Supports hybridization assays, FISH/ISH, PCR/qPCR probes, capture assays, biosensors and nanotechnology constructs.
Use the architecture selector to match arm count, branch complexity and label density to your assay objective.
Single branch point dividing into two arms, with balanced or asymmetric sequences.
Central node with three independent arms, variable arm lengths and optional labels.
High-density scaffold for four sequences, dyes, ligands or affinity groups.
Hierarchical branching to maximize valency, label count and scaffold complexity.
Branched oligonucleotides are useful when one linear probe cannot carry enough binding, reporting or capture function.
Branched constructs can carry multiple dyes or reporter modules on a single scaffold, increasing local signal without requiring more probes.
Different sequences on separate arms enable simultaneous hybridization or mutation-specific recognition.
Biotinylated multi-arm constructs can combine target binding and capture functions.
Multiple aptamer domains can increase avidity or enable crosslinking studies.
Defined branch points support DNA nanotechnology, biosensors and programmable assemblies.
A branched oligo quote is fastest when architecture, arm sequences, labels, spacer strategy, purification and QC needs are defined upfront.
Quote-ready file tip: Include a simple branch diagram or table showing each arm sequence, modification position, linker/spacer, scale, purification and QC requirement.
A simplified workflow keeps the page easy to scan while preserving the live-site ordering information.
Send arm layout, sequences, labels, branch point, linkers and intended application.
We optimize linkers, placement, branch chemistry and manufacturability.
Build multi-arm construct with method-matched purification and cleanup.
Release with MS, analytical HPLC, documentation and optional functional QC.
Fastest scoping: Attach diagrams, prior designs or an RFP. Include arm count, sequences, labels per arm, target Tm, scale, purification and QC requirements.
Branched constructs require confirmation of molecular identity, purity and label/arm integrity because multi-arm designs can produce complex analytical profiles.
QC packages may include MALDI or ESI mass spectrometry, analytical HPLC purity profile, multi-peak deconvolution, optional IE-HPLC or PAGE, and optional functional assays such as hybridization/Tm or capture efficiency.
MALDI or ESI MS supports molecular weight confirmation for the final branched construct.
Purity profile and multi-peak deconvolution where complex label or branch profiles are present.
Optional hybridization, Tm or capture efficiency testing for application-specific confidence.
Traceable documentation; RUO standard with GLP/cGMP support available on request.
Standard branched constructs: 2–3 weeks after order confirmation.
Multi-arm, multi-label or dendrimeric constructs may require additional time.
Rush options may be available depending on architecture, labels and purification.
Connect branched oligo designs with the right Bio-Synthesis synthesis, modification and analytical support services.
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Architecture, branch chemistry, linker strategy, label placement and manufacturability.
MS, analytical HPLC, optional PAGE/IE-HPLC and functional assay support.
Use this section to support scientific credibility while keeping the commercial page focused on architecture selection, synthesis feasibility, purification and analytical release.
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