Constrained Nucleotide Overview

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Custom Constrained Nucleotide Synthesis

Constrained nucleotides were first described by Wengel and co-workers in 1998 as a novel class of conformationally restricted oligonucleotide analogues. A constrained nucleotide is a bicyclic nucleic acid where a ribonucleoside is linked between the 2'-oxygen and the 4'-carbon atoms via a methylene unit.

Oligonucleotides containing Constrained Nucleotide exhibit unprecedented thermal stability towards complementary DNA and RNA2, which allows excellent mismatch discrimination. In fact, the high binding affinity of

Constrained Nucleotide oligos

is recommended for use in any hybridization assay that requires high specificity and/or reproducibility such as

All Constrained Nucleotide oligo synthesis include/are:

Labeled probes

In situ hybridization probe

SNP genotyping

DNA MicroArray expression analysis

Allele Specific PNA

mRNA sample preparation

Molecular Beacons and PCR primers

As a result of these significant characteristics, the use of Constrained Nucleotide-modified oligos in antisense drug development is now coming under investigation4 and, recently, the therapeutic potential of Constrained Nucleotide has been reviewed.⁵

BSI offers Constrained Nucleotide synthesis with great flexibility. For more information on

custom Constrained Nucleotide synthesis

, Contact us

Deprotected, desalted and ready to use

Multi-scale capacities

Purification by PAGE or RP-HPLC upon request

QC analysis by MALDI-TOF Mass spectrometry, HPLC, PAGE analysis

Fast delivery with high throughput capacity

References:

(1a) A.A. Koshkin, S.K. Singh, P. Nielsen, V.K. Rajwanshi, R. Kumar, M. Meldgaard, C.E. Olsen, and J. Wengel, Tetrahedron,1998, 54, 3607-3630.
(1b) S.K. Singh, P. Nielsen, A.A. Koshkin, and J. Wengel, Chem. Comm., 1998, (4), 455-456.
(2) L. Kværnø and J. Wengel, Chem. Comm., 1999, (7), 657-658.
(3) P. Mouritzen, A.T. Nielsen, H.M. Pfundheller, Y. Choleva, L. Kongsbak, and S. Møller, Expert Review of Molecular Diagnostics, 2003, 3(1), 27-38.
(4a) J. Kurreck, E. Wyszko, C. Gillen, and V.A. Erdmann, Nucleic Acids Res., 2002, 30, 1911-1918.
(4b)H. Ørum and J. Wengel, Curr. Opinion in Mol. Therap., 2001, 3, 239-243.
(5a) M. Petersen and J. Wengel, Trends in Biotechnology, 2003, 21(2), 74-81.
(5b) D.A. Braasch, D.R. Corey, Biochemistry, 2002, 41, 4503-4510.

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