Bio-Synthesis provides custom multiplex qPCR probes for simultaneous multi-target detection using spectrally separated fluorescent reporters, optimized quencher systems, and multiplex-compatible assay design strategies.
Multiplex qPCR uses multiple fluorescent probe sets in a single reaction to simultaneously detect several genetic targets across separate optical channels. Compared with singleplex qPCR, multiplex assays require more careful dye selection, primer/probe balancing, spectral overlap control, and instrument channel compatibility.
Reduce sample use, reagent use, and time by detecting multiple targets in the same tube or well.
Assign each target to a reporter dye that matches your instrument optics and expected signal level.
Primer and probe sets must be designed to avoid competition, crosstalk, and uneven Ct/Cq output.
Use channel planning to match reporter dyes with instrument filters, target abundance, and multiplex panel requirements. Wavelength values are approximate and should be confirmed against the dye supplier and qPCR instrument filter set.
Multiplex qPCR assays require coordinated optimization across reporter dyes, probe chemistry, primer interactions, optical channels, and amplification efficiency. As multiplex level increases, assay performance depends on managing spectral overlap, reaction competition, background fluorescence, and signal normalization across all targets.
A streamlined workflow for moving from target selection to a balanced, QC-supported multiplex qPCR probe panel without overloading the page with cards.
Target sequences, assay type, instrument channels, and multiplex level.
Dye assignment, quencher pairing, primer/probe balance, and overlap review.
QC-supported probes in tube, plate, pooled, normalized, liquid, or lyophilized formats.
Related qPCR probe services
Include targets, probe sequences if available, dye preferences, instrument channels, scale, purification, delivery format, and documentation needs.
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