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Custom Oligo Bioconjugation

Covalent conjugation of DNA/RNA to proteins, antibodies, peptides, lipids, and nanoparticles with precise control and rigorous QC.

Overview Advantages Applications Service Options Linker Chemistry Matrix Payloads & Partners QC How to Order FAQ Contact

ISO 9001:2015 / ISO13485:2016 45+ Years of Expertise U.S.A. Facilities-Texas GLP/GMP-Aligned

Overview

Bio‑Synthesis provides end‑to‑end oligonucleotide bioconjugation—from handle installation and linker selection to conjugation, purification, and orthogonal QC. We support DNA, RNA, and chemically modified backbones (2′‑OMe, 2′‑F, LNA, PS, PEGylated) and deliver conjugates ready for assays or product development.

Whether you need an antibody–oligo for proximity assays, a peptide–oligo for delivery, a lipid–oligo for uptake, or a nanoparticle–oligo for sensing, our chemistries and analytics ensure performance and reproducibility.

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At-a-Glance

  • Conjugation: protein/antibody, peptide, small molecule, lipid, polymer/PEG, nanoparticle, surface
  • Chemistry: NHS‑amine, EDC/NHS (carboxyl↔amine), maleimide‑thiol, SMCC, oxime/hydrazone, CuAAC/SPAAC, tetrazine‑TCO
  • DoL control: targeted degree of labeling with process‑adjusted stoichiometry
  • Purification: HPLC (RP/IE), size‑exclusion, desalting; PAGE on request
  • QC: MS, HPLC, UV/DoL calculation; optional ELISA/activity and functional assays
  • Scales: μg screening to gram‑scale production (RUO → GLP/cGMP on request)

Key Advantages

Broad Chemistry Toolbox

Orthogonal handles (amine, thiol, azide/alkyne, aldehyde, tetrazine/TCO) and crosslinkers for diverse biomolecules.

Precise DoL & Reproducibility

Process‑controlled stoichiometry and post‑purification analytics to meet your assay specifications.

End‑to‑End QC

Identity, purity, residual linker monitoring, and, when requested, activity/functional testing of conjugates.

Applications

  • Proximity ligation/extension assays (PLA/PEA)
  • Immuno‑PCR and immuno‑sequencing
  • In situ detection & imaging probes
  • Targeted delivery (peptide/lipid–oligo)
  • Surface capture & biosensors
  • Nanoparticle functionalization
  • Therapeutic research & MOE platforms
  • Barcode/UMI and NGS adapter strategies
  • Dual‑labeled probes and molecular beacons

Service Options

Parameter Options
Oligo Types DNA, RNA, 2′-OMe, 2′-F, LNA, PS; single- or double-stranded; aptamers; PNA/PMO on request
Handles (oligo) Amine, Thiol, Azide, Alkyne, DBCO/BCN, Aldehyde, Maleimide, Biotin, Digoxigenin
Partners Antibodies, proteins/enzymes, peptides/CPPs, small molecules, lipids (cholesterol, tocopherol), polymers/PEG, nanoparticles (Au, silica, QDs), surfaces
Stoichiometry Defined DoL (e.g., 1–3 oligos/antibody) or ratio ranges; site-selective strategies when available
Purification RP-HPLC, IEX-HPLC, size-exclusion, ultrafiltration/desalting; PAGE optional
Documentation COA with MS/HPLC/UV; optional activity/ELISA; GLP/cGMP documentation upon request

Linker Chemistry Matrix

Reactive Pair Typical Reagents Oligo ↔ Partner Notes & Common Uses
NHS-ester ↔ Amine NHS/TFP esters, SMCC/sulfo-SMCC (NHS side) Amine-oligo ↔ Lys/amine on protein/peptide Fast, robust; avoid Tris/primary amine buffers; pH 7.5–8.5.
Carboxyl ↔ Amine EDC (zero-length) ± NHS stabilization Carboxyl-oligo ↔ protein/peptide amine Forms amide; good for surfaces/polymers; requires control of pH and salt.
Maleimide ↔ Thiol SMCC, sulfo-SMCC; maleimide-PEG(n) Maleimide-oligo ↔ Cys/thiol on partner (or vice-versa) Site-selective via engineered Cys or reduced hinge; avoid excess reducing agents.
Thiol ↔ Thiol Disulfide bridges (DTNB), pyridyl-disulfide Thiol-oligo ↔ thiol-partner Cleavable linkers for triggered release; reversible in reducing environments.
Aldehyde/Ketone ↔ Hydrazide/Amine-oxy Hydrazone/oxime ligation Aldehyde-oligo ↔ hydrazide/aminooxy partner Chemoselective; can be NaBH3CN-stabilized; useful for glycan or formyl handles.
Azide ↔ Alkyne (CuAAC) Copper-catalyzed click Azide-oligo ↔ alkyne partner High yield, small scar; not copper-free—consider SPAAC when Cu-sensitive.
Azide ↔ DBCO/BCN (SPAAC) Strain-promoted click Azide-oligo ↔ DBCO/BCN partner No copper; excellent for antibodies/cells; slightly bulkier linkage.
Tetrazine ↔ TCO IEDDA click Tetrazine-oligo ↔ TCO-partner Bioorthogonal, very fast; great for sequential/orthogonal strategies.
Photocrosslinkers Diazirine, benzophenone Photo-oligo ↔ proximity partner For discovery or mapping interactions; forms covalent adducts upon light.

Payloads & Partners

Partner Type Examples Typical Chemistry Applications
Antibody / Fab IgG, Fab, scFv (customer‑supplied or sourced) Maleimide–thiol (reduced hinge or engineered Cys), SPAAC, tetrazine‑TCO PLA/PEA, immuno‑PCR, spatial proteomics, barcoded IHC
Proteins / Enzymes Streptavidin, enzymes, binding domains NHS‑amine, SPAAC, CuAAC, oxime/hydrazone Capture systems, reporters, proximity assays
Peptides / CPPs Arg‑rich CPPs, targeting peptides Maleimide–thiol, NHS‑amine, click Cell uptake, trafficking, targeting
Lipids Cholesterol, stearyl, tocopherol Amide coupling, click, thiol–maleimide Membrane anchoring, delivery enhancement
Polymers / PEG PEG(n), multi‑arm PEGs NHS‑amine, maleimide–thiol, click PK tuning, spacing, solubility
Nanoparticles / Surfaces Gold, silica, magnetic beads, sensors Thiol–Au, silane chemistry, NHS‑amine, click Diagnostics, enrichment, biosensing
Small Molecules / Haptens Biotin, DIG, fluorophores, dyes NHS‑amine, click, oxime/hydrazone Detection, capture, imaging
We offer much more than listed! - get in touch   
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Quality Assurance

  • Mass spectrometry and analytical HPLC for oligo identity/purity
  • Conjugate QC: SEC‑HPLC or SDS‑PAGE (as applicable), UV‑Vis DoL, residual linker check
  • Optional: ELISA/activity, binding/affinity, functional assay support
  • Documentation: RUO by default; GLP/cGMP documentation on request

Typical Turnaround

Standard peptide/protein conjugates: 2–3 weeks from order confirmation. Antibody conjugates and multi‑step builds may require additional time.

Lead time depends on partner source/quality, chemistry, and DoL specification; rush options may be available.

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How to Order

  1. Share oligo sequence(s) and desired handles (or request design assistance).
  2. Specify partner type (antibody/protein/peptide/lipid/polymer/nanoparticle) and preferred chemistry.
  3. Choose scale, purification, and QC/functional tests.
  4. Receive a same‑day quote and projected timeline.

Supplying your own protein/antibody? Include concentration, buffer composition, and storage conditions.

Design Checklist

  • Oligo length/type and modification sites
  • Partner characteristics (MW, pI, buffer, reactive groups)
  • Targeted DoL and site‑selectivity (if applicable)
  • Assay context, storage buffer, and shipping conditions

FAQ

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