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ANIMALS:
Two New Zealand white Males, approximately 6 months, approximately 5-6 lbs in weight
ADJUVANTS:
Primary Injection:
(1:1) 0.2 mg of conjugated peptide (app. 5ml of conjugate solution) Plus equal amounts of Freund's complete
Booster Injection:
(1:1) 0.2 mg of conjugated peptide (app. 5ml of conjugate solution) Plus equal amount of Freund's Incomplete
STANDARD SCHEDULE
Day |
Procedure |
Amounts/Volumes per Rabbit |
0
(week 0) |
Pre-bleed
Primaray Injuection |
2.0 - 5.0 ml of SERUM (1:1) conjugate solution: Freund's Complete |
14
(week 2) |
First - Booster |
(1:1) conjugate solution: freund's Incomplete |
28
(week 4) |
second - Booster |
(1:1) conjugate solution: Freund's Incomplete |
42
(week 6) |
First Production bleed
Third Booster perform |
20 ml of SERUM
(1:1) conjugate solution: Freund's Incomplete |
56
(week 8) |
Second Production Bleed
Fourth Booster
ELISA |
20 ml of SERUM
(1:1) conjugate solution: Freund's Incomplete |
70
(week 10) |
Third Production Bleed |
20ml of SERUM |
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At the end of the 70 day protocol, the researcher is contacted to determine:
1. Continue project on a month-to-month basis
2. Terminate Project
3. Project exsanguination
Call 800-227-0627 or write to us at biosyn@biosyn.com for details |
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