BCN-PEG12
BCN-PEG12 is a premium bioorthogonal click chemistry modification designed for efficient conjugation of oligonucleotides to proteins, antibodies, nanoparticles, polymers, and other biomolecules under mild, catalyst-free conditions. The modification combines a highly reactive bicyclo[6.1.0]nonyne (BCN) cyclooctyne with an extended PEG12 (dodecaethylene glycol) spacer, providing exceptional flexibility, excellent aqueous solubility, and significant separation between the oligonucleotide and its attached payload.
The BCN group reacts rapidly and selectively with azide-functionalized molecules through strain-promoted azide-alkyne cycloaddition (SPAAC), producing a stable triazole linkage without requiring copper catalysis. Because no metal catalyst is needed, BCN-PEG12 is particularly well suited for conjugating sensitive biomolecules, therapeutic oligonucleotides, living cells, and biological samples where copper ions may reduce activity or induce toxicity.
Compared with BCN, BCN-PEG2, BCN-PEG3, BCN-PEG4, and BCN-PEG8, the PEG12 spacer provides the greatest distance between the oligonucleotide and the conjugated molecule. This longer hydrophilic spacer substantially reduces steric hindrance, improves accessibility of the BCN group, enhances conjugation efficiency with bulky biomolecules such as antibodies and enzymes, and helps preserve oligonucleotide hybridization and biological activity following conjugation. The PEG12 linker also improves water solubility while reducing nonspecific adsorption and aggregation.
BCN-PEG12 reacts efficiently with a wide range of azide-containing molecules, including Azido C6, Azido PEG linkers, azide-modified peptides, proteins, antibodies, fluorescent dyes, lipids, glycans, drug molecules, polymers, and nanoparticles. The resulting triazole linkage is chemically robust, biologically stable, and compatible with physiological buffers, making BCN-PEG12 an excellent choice for advanced bioconjugation and therapeutic research.
Bio-Synthesis offers custom BCN-PEG12-modified oligonucleotides for DNA, RNA, PNA, LNA, siRNA, antisense oligonucleotides (ASOs), aptamers, molecular beacons, CRISPR guide RNAs, and other synthetic nucleic acids. BCN-PEG12 may be incorporated at the 5′ terminus, 3′ terminus, or selected internal positions and is compatible with numerous additional modifications including fluorophores, quenchers, biotin, thiol modifiers, amino modifiers, GalNAc, cholesterol, PEG linkers, peptide conjugates, antibody conjugates, lipid conjugates, polymers, and nanoparticle conjugates.
Typical applications include:
- Copper-free SPAAC click chemistry
- Antibody-oligonucleotide conjugates (AOCs)
- Protein-oligonucleotide conjugates
- Peptide conjugation
- Fluorescent dye conjugation
- PEGylated bioconjugates
- Nanoparticle functionalization
- Aptamer conjugation
- DNA nanotechnology
- Therapeutic oligonucleotide research
Each modified oligonucleotide is synthesized using high-quality solid-phase phosphoramidite chemistry and is available with optional HPLC or PAGE purification. Comprehensive analytical characterization includes HPLC, LC-MS, MALDI-TOF, UV spectroscopy, and additional quality control testing to verify sequence identity, purity, and successful incorporation of the BCN-PEG12 modification.
| Property |
Typical Value |
| Modification |
BCN-PEG12 |
| Reactive Group |
BCN (Bicyclo[6.1.0]nonyne) |
| Spacer |
PEG12 (Dodecaethylene glycol) |
| Click Chemistry |
SPAAC (Copper-free) |
| Reaction Partner |
Azide-functionalized molecules |
| Catalyst Required |
None |
| Typical Attachment |
5′ end, 3′ end, or internal modification |
| Compatible Oligos |
DNA, RNA, PNA, LNA, siRNA, ASO, Aptamers |
| Typical Applications |
SPAAC conjugation, antibody conjugation, protein conjugation, PEGylation, nanoparticle functionalization, fluorescent labeling |
Product Information
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Jewett JC, Bertozzi CR. Cu-free click cycloaddition reactions in chemical biology. Chemical Society Reviews. 2010;39:1272-1279. DOI: 10.1039/B901970G
- Dommerholt J, Rutjes FPJT, van Delft FL. Strain-promoted 1,3-dipolar cycloaddition of cycloalkynes and organic azides. Topics in Current Chemistry. 2016;374:16. DOI: 10.1007/s41061-016-0039-6
- Sletten EM, Bertozzi CR. Bioorthogonal chemistry: Fishing for selectivity in a sea of functionality. Angewandte Chemie International Edition. 2009;48(38):6974-6998. DOI: 10.1002/anie.200900942
- Hermanson GT. Bioconjugate Techniques. 3rd ed. Academic Press; 2013.
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