Purification and crystallization of Vibrio fischeri CcdB and its complexes with fragments of gyrase and CcdA
Natalie De Jonge et al.
12/01/2013
Acta Cryst. (2007). F63, 356–360
The ccd toxin–antitoxin module from the Escherichia coli F plasmid has a homologue on the Vibrio fischeri integron. The homologue of the toxin (CcdBVfi) was crystallized in two different crystal forms. The first form belongs to space group I23 or I213, with unit-cell parameter α = 84.5 Å, and diffracts to 1.5 Å resolution. The second crystal form belongs to space group C2, with unitcell parameters = a = 58.5, b = 43.6, c = 37.5 Å, β = 110.0°, and diffracts to 1.7 Å resolution. The complex of CcdBVfi with the GyrA14Vfi fragment of V. fischeri gyrase crystallizes in space group P212121, with unit-cell parameters a = 53.5, b = 94.6, c = 58.1 Å, and diffracts to 2.2 Å resolution. The corresponding mixed complex with E. coli GyrA14Ec crystallizes in space group C2, with unit-cell parameters a = 130.1, b = 90.8, c = 58.1 Å, β = 102.6°, and diffracts to 1.95 Å . Finally, a complex between CcdBVfi and part of the F-plasmid antitoxin CcdAF crystallizes in space group P212121, with unit-cell parameters a = 46.9, b = 62.6, c = 82.0 Å , and diffracts to 1.9 Å resolution.