Clicky

Custom Cell Line Identification
Cell Line Validation
Human Cell Line Identification and Authentication Services
The availability of well-characterized, viable human tissues and cell lines accompanied by pertinent donor data is a prerequisite for a great deal of current biomedical and transplantation research. Unfortunately, the misidentification and cross-contamination of human tissue cell lines occurs frequently. It is estimated that over 20% of all established cell lines are misclassified by cell type or species of origin, and over 33% of all cell lines suffer inter- or intra-species cross-contamination (1-3). The ATCC, along with the Coriell Institute for Medical Research, the European Collection of Cell Cultures (ECACC), the Deutsche Sammlung von Mikrorganismen and Zellkulturen (DSMZ), and the Japanese Collection of Research Resources have all received cell line submissions that, upon authentication, were determined to have been misidentified by the depositor (4,5). This poses a serious threat to the legitimacy of research findings produced from any of these cell cultures.

For a list of misidentified or contaminated cell lines.

To aid clients in need of cell line identity validation, Bio-Synthesis (BSI) offers fast and reliable human cell line identification services to authenticate your cell lines. Our cell line identification laboratory performed DNA Idenfication at accredited DNA testing facilities by scientists with experience in STR DNA profiling, SNP genotyping, high-resolution Luminex HLA DNA typing, tissue microarray analysis and bioinformatic data analyses. Our commitment to total quality management assures that our scientists employ efficient methods to confirming species' identity and detect cross-contamination, authenticating our client's cell lines in full compliance with NIH recommendations and scholarly journal guidelines.
Cell Line Identification Service Packages
  Standard Premium Gold Custom
$120 $300 $550 Custom
DNA Extraction
Unknown Sample Profiling
Known Reference Profiling  
Comparison Analysis  
Bioinformatics Data Analysis    
Electropherogram & Notarization    
 
Standard: Cell Culture STR Profiling (Cat. No.: CL1002) $120/sample
Service Description (3-5 days)
DNA Extraction: DNA extraction from standard cell line samples (Dry Cell Pellets, Frozen Cell Pellets and Cryopreservation Tube)
Unknown Sample Profiling: Short tandem repeat (STR) profiling of a cell line sample using AmpFℓSTR® Identifiler® for high resolution screening and intra-species cross-contamination detection.
 
 
Premium: Cell Culture STR Profiling and Comparison Analysis (Cat. No.:CL1003) $300/sample
Service Description (3-5 days)
DNA Extraction: DNA extraction from standard cell line samples (Dry Cell Pellets, Frozen Cell Pellets and Cryopreservation Tube)
Unknown Sample Profiling: Short tandem repeat (STR) profiling of a cell line sample using AmpFℓSTR® Identifiler® for high resolution screening and intra-species cross-contamination detection.
Known Reference Profiling: Short tandem repeat (STR) profiling of a cell line sample using AmpFℓSTR® Identifiler® for high resolution screening and intra-species cross-contamination detection or a database lookup of a known cell line profile at one of the recognized repositories (ATCC).
Comparison Analysis: Comparison study of the known and unknown samples to determine whether the profiles match or if any contamination or mutations have occurred relative to the known sample.
Bioinformatics Data Analysis: Database search and analysis to identify cell origin of unknown sample with the closest matches, and any drift in genotype using three recognized repositories (ATCC, DSMZ, and JCRB). The sample contributor will be advised of any resulting matches in the certificate of analysis.
 
 
Gold: Cell Culture STR Profiling, Comparison, and Bioinformatic Data Analysis (Cat. No.: CL1004) $550/sample
Service Description (3-5 days)
DNA Extraction: DNA extraction from standard cell line samples (Dry Cell Pellets, Frozen Cell Pellets and Cryopreservation Tube)
Unknown Sample Profiling: Short tandem repeat (STR) profiling of a cell line sample using AmpFℓSTR® Identifiler® for high resolution screening and intra-species cross-contamination detection.
Known Reference Profiling: Short tandem repeat (STR) profiling of a cell line sample using AmpFℓSTR® Identifiler® for high resolution screening and intra-species cross-contamination detection or a database lookup of a known cell line profile at one of the recognized repositories (ATCC, DSMZ, and JCRB).
Comparison Analysis: Comparison study of the known and unknown samples to determine whether the profiles match or if any contamination or mutations have occurred relative to the known sample.
Bioinformatics Data Analysis: Database search and analysis to identify cell origin of unknown sample with the closest matches, and any drift in genotype using three recognized repositories (ATCC, DSMZ, and JCRB). The sample contributor will be advised of any resulting matches in the certificate of analysis.
Electropherogram: An original electropherogram will be provided to give you the exact intensities of each allele detected.
Notarization: Notarization of certificate of analysis will be provided.
 
 
Create Your Own Identification and Validation Packages
Customize Detection Systems for Profiling  
AmpFℓSTR® Identifiler® $95/sample
PowerPlex® 1.2 $150/sample (Min 20 samples)
PowerPlex® 16HS $150/sample (Min 20 samples)
StemEliteTM $150/sample (Min 20 samples)
High-Resolution Luminex HLA DNA Genotyping (Extended Cell Line Identification): This high-resolution HLA analysis is used for populations that interbreed closely. Read more about high-resolution HLA analysis in cases of STR marker depletion. $200/sample (Min 10 samples)
 
Additional Services  
DNA Extraction: DNA extraction from standard cell line samples (Dry Cell Pellets, Frozen Cell Pellets and Cryopreservation Tube)
We offer volume discounts for DNA extraction, please inquire.
$25/sample
Non-Standard DNA Extraction: DNA extraction from non-standard samples (Blood, Slides and etc.)
We offer volume discounts for DNA extraction, please inquire.
$250/sample
Comparison Analysis: Comparison study of the known and unknown samples to determine whether the profiles match or if any contamination or mutations have occurred relative to the known sample. $100
Profile Search: Using only the name or ATCC number of your cell line, we can accurately find its DNA profile in recognized repositories (ATCC, DSMZ, and JCRB). $150/cell line
Bioinformatics Data Analysis: Database search and analysis to identify cell origin of unknown sample with the closest matches, and any drift in genotype using three recognized repositories (ATCC, DSMZ, and JCRB). The sample contributor will be advised of any resulting matches in the certificate of analysis. Cell line profile must be provided. $250/profile
Species-Specifics Authentication: Our species-specifics authentication services can selectively verify whether the cell line in question is indeed one of the 14 detectable species using our two-pronged inter-species identification and detection approach. Intra-species identification services is currently only available for human cell lines.
(Detectable Species: Pig, Human, Cat, Chinese hamster, Rhesus monkey, Sheep, Horse, African green monkey, Rat, Dog, Mouse, Rabbit, Goat, and Bovine)

$250/species

or

$1000 for all 14 species

Electropherogram: The original electropherogram will be provided to give you the exact intensities of each allele detected. $50/sample
Notarization: Notarization of certificate of analysis will be provided. $30/service packages
Consulting and other additional fees may apply  
Reference:
1. Drexler, H.G., Dirks, W.G. and MacLeod, R.A.F. (1999) Leukemia 13, 1601–7.
2. Drexler, H. G. et al. (2001) Blood 98, 3495–6.
3. Cabrera, C.M. et al. (2006) Cytotechnology 51, 45–50.
4. Chatterjee, R. (2007) Science 315, 928–31.
5. MacLeod, R.A.F. et al. (1999) Int. J. Cancer 83, 555–63.