Sterol CholesterolPrimary sterol platform Commercial Direct3′ SupportPost-Conjugation 5′ • 3′ • internal/custom+ Direct incorporation Established cholesterol modifier phosphoramidite and cholesterol-bearing solid-support formats can provide defined terminal architectures. Post-conjugation Reactive oligonucleotide handles allow custom cholesterol attachment, alternative spacers, internal positions, or multi-modification constructs. Applications siRNA and ASO delivery research, membrane anchoring, lipoprotein-interaction research, imaging, and specialty lipid-oligo assemblies. Design watch Linker length, strand, position, hydrophobicity, purification, and biological context should be optimized together.
Sterol Cholesteryl-TEGCholesterol with triethylene glycol spacer Commercial PhosphoramiditeCustom Conjugation Possible Typically terminal; architecture dependent+ Architecture Cholesterol is separated from the oligonucleotide by a flexible TEG spacer, reducing direct steric crowding compared with a compact linkage. Use Useful for uptake, membrane-association, siRNA/ASO, aptamer, and dual-modification designs where a hydrophilic spacer is desirable.
Bile Acid Cholic AcidSteroidal bile acid Published DirectPost-ConjugationCustom 5′ published • 3′/internal custom+ Direct incorporation Cholic-acid phosphoramidite chemistry has published precedent for solid-phase oligonucleotide synthesis. Conjugation Post-synthetic coupling can be used when different linkers, positions, or oligonucleotide architectures are required. Design role Amphiphilic steroidal scaffold for sterol/bile-acid SAR and transport-oriented research. Commercial status Published direct chemistry should not be interpreted as routine commercial availability or guaranteed project feasibility.
Plant Sterol BrassicasterolUnsaturated phytosterol Post-ConjugationCustom Building Block 5′ • 3′ • selected internal/custom+ Recommended route Post-synthetic conjugation is the primary website classification unless a project-specific direct-incorporation reagent is developed and validated. Design use Useful for comparing side-chain and double-bond differences across sterol-oligonucleotide SAR panels.
Plant Sterol CampesterolPhytosterol Post-ConjugationCustom Building Block 5′ • 3′ • selected internal/custom+ Recommended route Post-synthetic conjugation or project-specific modifier development can be selected based on position, linker, scale, and sterol reagent format. Design use Plant-sterol comparison against cholesterol, sitosterol, and stigmasterol.
Plant Sterol β-SitosterolPhytosterol Post-ConjugationCustom Building Block 5′ • 3′ • selected internal/custom+ Recommended route Post-synthetic conjugation is the primary standard classification; direct-incorporation chemistry can be reviewed as a custom development project. Design use Hydrophobic sterol SAR, membrane-association research, and comparison with cholesterol-based conjugates.
Plant Sterol StigmasterolUnsaturated phytosterol Post-ConjugationCustom Building Block 5′ • 3′ • selected internal/custom+ Recommended route Post-synthetic conjugation with project-specific linker and reactive-handle selection. Design use Useful for sterol side-chain and unsaturation comparisons in structure-activity studies.
Sterol Additional SterolsErgosterol, cholestanol and custom analogs Post-ConjugationCustom Development Architecture dependent+ Custom scope Additional sterols can be evaluated based on available functional groups, linker strategy, reagent stability, and final oligonucleotide architecture. Quote requirement Exact sterol structure, desired attachment position, linker, sequence, scale, and analytical package should be defined before route selection.