40+ years of excellence in custom synthesis and bioconjugation services.
Calculators, design tools, and educational content to support your research.
Design and manufacture DNA, RNA, PNA, branched DNA, capture, telomere and multiplex hybridization probes with flexible labeling, purification and analytical quality-control options.
Hybridization probes are sequence-specific oligonucleotides that bind complementary DNA or RNA targets. They can generate a signal, isolate a target, increase detectable signal through layered architectures, or provide high-affinity recognition for demanding sequences.
Bio-Synthesis supports projects ranging from a single standard DNA or RNA probe to advanced PNA designs, branched DNA signal-amplification systems, capture probes, telomere probes and coordinated multiplex probe sets.
Probe chemistry, length, melting temperature, target accessibility, label placement, purification and QC should be considered as one connected design—not as separate purchasing decisions.
The portfolio is organized by function: routine sequence recognition, higher-affinity binding, signal amplification, target capture, specialized repeat analysis and custom multi-probe panels.
General-purpose probes for routine detection, blotting, verification and research hybridization.
Explore →
Select the outcome you need. The guide connects common project goals with the technology most likely to deserve initial evaluation.
A standard DNA probe is often the most practical starting point for genomic DNA, plasmids, PCR products or synthetic DNA targets.
Southern blotting, dot blotting, genomic target verification, pathogen research and general DNA detection.
DNA or RNA chemistry can be selected according to duplex requirements, nuclease conditions, assay format and preferred detection strategy.
Northern blotting, viral RNA detection, transcript analysis, RNA capture and low-copy target studies.
Capture probes are designed around efficient target enrichment, controlled nonspecific binding and compatibility with the immobilization platform.
Hybrid capture, pull-down assays, NGS target enrichment, biomarker isolation and target depletion.
PNA or selected affinity-enhanced chemistries may improve recognition of structured, repetitive, high-GC or mismatch-sensitive targets.
Mutation analysis, repeat-sequence detection, high-GC targets, allele discrimination and difficult hybridization regions.
Signal-amplification systems can increase readout from low-abundance targets while avoiding enzymatic amplification of the target itself.
Low-copy RNA, gene-expression analysis, sensitive target detection and multiplex signal amplification.
Multiplex panels require coordinated sequence design, compatible Tm windows, cross-reactivity review, label balance and normalization.
Pathogen panels, multi-gene studies, transcript profiling, genomic panels and custom target collections.
Specialized probes are optimized for telomeric repeats and chromosome-end analysis, often using high-affinity PNA chemistry.
Telomere length studies, chromosome biology, cytogenetic research and repeat-sequence analysis.
The comparison below summarizes the strengths of each hybridization probe technology. Final selection should consider target sequence, hybridization conditions, detection platform and analytical requirements.
Use the controls below to generate a general starting recommendation for chemistry, length, label, purification, QC and design focus.
A standard DNA oligonucleotide is a practical starting format for routine sequence-specific DNA detection.
Each project is unique. Final probe chemistry, labeling strategy, purification method and analytical QC should be selected based on the target sequence, assay workflow and performance requirements.
Probe architecture determines how the target is recognized and how detection, capture or signal amplification is achieved.
Double-stranded target recognition by a complementary DNA probe.
RNA-focused duplex format for transcript and viral RNA targets.
Neutral-backbone, high-affinity duplex architecture.
Duplex-forming probe with a fluorescent or hapten label.
Affinity-tagged duplex for target enrichment or isolation.
Branched architecture supporting layered signal amplification.
The application should guide chemistry, architecture and label selection. The same target may require a different probe design depending on whether the goal is detection, capture, amplification or multiplex analysis.
Sequence-specific recognition for genomic, plasmid, synthetic and pathogen-derived DNA targets.
Probe designs for transcript detection, viral RNA, expression studies and RNA-focused research assays.
Affinity-tagged probes and custom panels for target enrichment, isolation and sequencing workflows.
Advanced designs for difficult sequences, low-abundance targets, repeat regions and multiplex studies.
Length and composition influence duplex stability, kinetics, mismatch discrimination and assay stringency. Multiplex probe sets should be designed toward compatible Tm windows.
Target folding can reduce probe access, especially for RNA. Accessible regions or multiple independent probe sites may improve performance.
Sequences should be reviewed for paralogs, isoforms, repeats, off-target complementarity and organism-specific background.
Fluorophores, biotin, DIG, DNP and reactive handles can be placed at the 5′ end, 3′ end or internally when compatible with the design.
Sequence, chemistry, label placement, purification and analytical strategy should be reviewed as one connected program.
Custom biomolecule synthesis experience
DNA, RNA, PNA, bDNA, capture and custom panels
Design review through analytical release
Target-region selection, sequence review, Tm balancing, accessibility and panel-level optimization.
DNA, RNA, PNA, affinity-enhanced analogs, fluorophores, affinity tags and reactive handles.
Individual, normalized, pooled and multiplex probe-set manufacturing with custom delivery formats.
Purity, identity, quantitation and label-specific characterization selected according to molecular complexity.
Documented workflows, traceable materials, controlled production and fit-for-purpose analytical release support custom hybridization probe projects.
Move directly to the specialized probe service aligned with your design.
Hybridization probe projects may extend into imaging and spatial biology.
Trusted by biotech leaders worldwide for over 45+ years of delivering high quality, fast and scalable synthetic biology solutions.