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Protein Carrier Conjugation

Bio-Synthesis offers conjugation of peptides to a variety of immunogenic carrier proteins and gel for use as immunogen or for affinity purification. A variety of immunogenic carrier proteins such as Keyhole Limpet Hemocyanin (KLH), Diphtheria toxoid has been use in conjugation with several conjugation chemistries. We also offer coupling of peptide to various types of gel to create an immunosorbent, for positive or negative selection of macromolecular binding entries, such as antibodies.

Carrier Protein

Description		Price
	KLH - keyhole lympet hemocyanin	$200.00
	BSA - bovine serum albumin	$200.00
	OVA - ovalbumin	$200.00
	THY - thyroglobulin	$200.00
	MAP - multiple antigen peptide (no conjugation necessary)	$150.00
	Other protein conjugation	Inquire

Serum production packages also available with large selection of host animals, click here to explore more on our custom peptide antibody production.

:Carrier: Keyhole Limpet Hemocyanin (KLH), Bovine Serum Albumin (BSA), Rabbit Serum Albumin (RSA), Ovalbumin, Thyroglobulin, or Human Gamma Globulin (HGG). Other proteins are available upon request.
Cross-Links: MBS (and other bi-functional linkers), which couple through sulfhydryl groups, and glutaraldehyde, which couples primarily through alpha, sigma & epsilon amino groups

* Amino Acid Analysis of Conjugates available upon request

References:

[1] Lee, A.C., Powell, J.E., Tregear, G.W., Niall, H.D. and Stevens, V.C. (1980) A method for preparing b-hCG COOH peptide-carrier conjugates of predictable composition. Mol. Immunol. 17; 749-756.

[2] Posnett, D., McGrath, H., and Tam, J.P. A novel method for producing anti-peptide antibodies, The Journal of Biological Chemistry, 263, 1719-1725 (1988),

[3] Tam, J.P., Synthetic peptide vaccine design: synthesis and properties of a high-density multiple antigenic peptide system, Proc. Natl. Acad, Sci., 85, 5409-5413 (1988).

[4] Wang, C. Y., Looney, D.J., Li, M.L., Walfield, A.M., Ye, J., Hosein, B., Tam, J.P. and Wong-Staal, F., Long-term high-titer neutralizing activity induced by octameric synthetic HIV antigen, Science, 254, 285-288. (1991)

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