 |
Biosyn
has refined Amino Acid Analysis methodologies ensuring accurate and
reliable results from a broad range of sample types including: food
stuffs, traditional medicines, recombinant protein sources & plant
materials.
Amino acid analysis (AAA) is a classic analytical technique that
characterizes proteins and peptides based on the composition of
their constituent amino acids. It provides qualitative
identification and is essential for the accurate quantification of
proteinaceous materials. Amino acid analysis is widely applied in
research, clinical facilities, and industry. |
|
|
|
| |
 |
|
Amino Acid Analysis |
|
| |
Amino Acid
Analysis is one of the best quantitative methods in protein
chemistry and used by many laboratories for peptide/protein
characterization. Whilst a well established technique in
protein chemistry, complications can come from a variety of
sources including: variability in the inherent lability of
amino acids to hydrolysis, oxidation and modification,
variability in instrument performance and hydrolysis/derivatisation
conditions, background or sample contamination,
datareduction methods, work habits. |
|
|
|
|
| |
 |
|
Steps Involved In Amino Acid Analysis |
|
| |
Amino acid
analysis involves four basic steps: hydrolysis (or
deproteination with physiological samples), separation,
derivatization, and detection.First, Hydrolyze a protein
to individual constituent amino acids; Second, Label amino
acids with a detectable UV-absorbing or fluorescent
marker;Third, Separate different types of amino acids by
chromatography; Fourth, Measure relative amounts of each
amino acid type based on intensity of the detectable
marker associated |
|
|
|
| |
|
|
