2'-Amino-Cytidine (2'-Amino-C)
2'-Amino-Cytidine (2'-Amino-C) is a modified cytidine nucleoside in which the 2'-hydroxyl group of the ribose sugar is replaced with a primary amino (-NH₂) group. This structural modification introduces a chemically reactive functionality while preserving the nucleobase, enabling the synthesis of oligonucleotides with unique physical, chemical, and biological properties. Because the amino group projects from the sugar rather than the nucleobase, it can serve as both a structural modification and a reactive handle for further chemical derivatization.
Compared with unmodified RNA, incorporation of 2'-Amino-C can influence duplex stability, alter local sugar conformation, and improve resistance to nuclease degradation under certain conditions. The presence of the 2'-amino functionality also provides opportunities for additional post-synthetic conjugation through amide formation, acylation, or other amine-selective chemistries, allowing researchers to introduce fluorophores, affinity tags, crosslinkers, or other functional molecules at precisely defined positions within an oligonucleotide.
Unlike terminal amino modifiers, which are typically attached through linker arms at the 5' or 3' ends, 2'-Amino-C places the amino group directly on the sugar backbone within the oligonucleotide sequence. This enables site-specific modification while maintaining the overall architecture of the nucleic acid, making it valuable for mechanistic studies and the development of specialized nucleic acid constructs.
Typical applications include:
- RNA structure and function studies
- Site-specific chemical derivatization
- Post-synthetic fluorescent labeling
- Bioconjugation with NHS esters and other amine-reactive reagents
- Crosslinking studies
- Antisense oligonucleotide research
- Aptamer engineering
- RNA-protein interaction studies
- Nucleic acid nanotechnology
- Molecular biology and synthetic biology research
Bio-Synthesis offers 2'-Amino-Cytidine incorporation into custom RNA, chimeric DNA/RNA oligonucleotides, antisense oligonucleotides (ASOs), siRNA, aptamers, and other synthetic nucleic acids. The modification can be incorporated at user-defined positions and combined with a broad range of additional chemistries, including fluorophores, quenchers, biotin, PEG linkers, click chemistry handles, peptides, GalNAc conjugates, lipids, and other custom modifications to support diverse research applications.
Each oligonucleotide containing 2'-Amino-C is synthesized using optimized solid-phase phosphoramidite chemistry and is available with optional desalting, HPLC purification, or PAGE purification, together with analytical characterization by LC-MS, MALDI-TOF, capillary electrophoresis, or other quality control methods, depending on project requirements.
Because it combines the hybridization properties of a modified RNA nucleotide with the synthetic flexibility of a reactive primary amine, 2'-Amino-Cytidine is a versatile building block for designing chemically functionalized oligonucleotides used in diagnostics, therapeutics, molecular imaging, and advanced nucleic acid research.
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Product Information
2'-Amino-Cytidine (2'-Amino-C)
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
Adler, A.; Forster, N.; Homann, M.; Goringer, H.U. Post-SELEX chemical optimization of a trypanosome-specific RNA aptamer. Comb. Chem. High Throughput Screen 2008, 11, 16–23.
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