2'-Amino-Uridine (2'-Amino-U)
2'-Amino-Uridine (2'-Amino-U) Oligonucleotide Modification
2'-Amino-Uridine (2'-Amino-U) is a modified RNA nucleoside in which the 2'-hydroxyl (2'-OH) group of the ribose sugar is replaced by a primary amino (-NH₂) group. This modification introduces a chemically reactive functionality directly into the RNA backbone while preserving the uracil nucleobase and its ability to participate in standard Watson-Crick base pairing with adenine. The presence of the 2'-amino group provides an excellent platform for site-specific conjugation and functionalization of synthetic RNA oligonucleotides.
Unlike conventional terminal amino modifiers that introduce reactive groups through linker arms at the 5' or 3' ends, 2'-Amino-Uridine places the primary amino group within the oligonucleotide sequence. This enables researchers to install fluorescent dyes, affinity labels, crosslinkers, peptides, nanoparticles, or other functional molecules at precisely defined internal positions while minimizing changes to the overall architecture of the nucleic acid.
The introduction of the amino functionality may also influence local sugar conformation, duplex stability, and nuclease susceptibility, making 2'-Amino-U valuable for studying RNA structure, molecular recognition, and RNA-protein interactions. Because the modification is incorporated during solid-phase synthesis, it provides a reproducible method for generating site-specifically modified RNA molecules for biochemical and biophysical investigations.
Common post-synthetic conjugation chemistries include reactions with NHS esters, isothiocyanates, activated carboxylic acids, and other amine-selective reagents, allowing efficient attachment of fluorophores, biotin, digoxigenin, PEG linkers, photoaffinity labels, click chemistry handles, and numerous other functional groups.
Typical applications include:
- Site-specific RNA labeling
- Fluorescent probe development
- RNA bioconjugation
- Crosslinking studies
- RNA structural biology
- RNA-protein interaction studies
- Antisense oligonucleotide research
- Aptamer engineering
- Molecular imaging
- RNA nanotechnology
- Synthetic biology
Bio-Synthesis offers 2'-Amino-Uridine incorporation into custom RNA oligonucleotides, chimeric DNA/RNA constructs, antisense oligonucleotides (ASOs), siRNA, aptamers, and other synthetic nucleic acid formats. The modification can be incorporated at user-defined positions and combined with a wide variety of additional chemistries, including fluorophores, quenchers, biotin, PEG linkers, GalNAc, lipids, click chemistry handles, peptides, and other custom conjugates.
Each oligonucleotide is synthesized using optimized phosphoramidite chemistry and is available with optional desalting, HPLC purification, or PAGE purification, together with analytical characterization by LC-MS, MALDI-TOF, UV spectroscopy, or other quality control methods as required.
The combination of sequence-specific incorporation, chemical versatility, and site-selective conjugation capability makes 2'-Amino-Uridine an important modification for RNA engineering, molecular diagnostics, therapeutic oligonucleotide development, and advanced nucleic acid research.
Product Information
2'-Amino-Uridine (2'-Amino-U)
2'-Amino-U, 2'-Amino-Uridine
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.
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