2'-Deoxyisocytidine, Iso-dC
2′-Deoxyisocytidine (iso-dC) is an artificial pyrimidine nucleoside in which the hydrogen-bonding pattern of natural cytidine has been rearranged to create a non-natural base. When incorporated into DNA, iso-dC typically pairs with 2′-deoxyisoguanosine (iso-dG), forming an orthogonal base pair that is distinct from the natural A:T and G:C base pairs. This alternative pairing system provides an important platform for expanding the chemical and informational diversity of synthetic nucleic acids.
The iso-dC:iso-dG base pair has been extensively investigated as one of the earliest artificial base-pairing systems for studying alternative genetic alphabets, DNA recognition, and nucleic acid engineering. Although it is not part of the modern Benner dZ:dP expanded genetic alphabet, iso-dC remains an important research tool for exploring hydrogen-bonding patterns, polymerase recognition, duplex stability, and molecular evolution.
Synthetic oligonucleotides containing iso-dC are widely used in synthetic biology, DNA nanotechnology, aptamer development, structural biology, polymerase engineering, and molecular diagnostics. The modification enables researchers to investigate non-natural base pairing while preserving the overall geometry of the DNA duplex.
Bio-Synthesis offers custom DNA oligonucleotides containing 2′-deoxyisocytidine synthesized using high-quality phosphoramidite chemistry. iso-dC may be incorporated at user-defined positions within DNA sequences and combined with numerous additional modifications, including fluorophores, quenchers, biotin, amino modifiers, thiol modifiers, click chemistry handles, PEG linkers, and phosphorothioate linkages.
Typical applications include:
- Artificial base pairing
- Synthetic biology
- DNA nanotechnology
- Aptamer engineering
- Molecular diagnostics
- Polymerase engineering
- DNA structural biology
- Nucleic acid chemistry
- Orthogonal recognition systems
- Expanded genetic alphabet research
Each modified oligonucleotide is manufactured under stringent quality standards and is available with optional HPLC or PAGE purification, together with analytical characterization by LC-MS, MALDI-TOF, capillary electrophoresis, and additional quality control testing to verify product identity and purity.
Research Use Only (RUO): iso-dC-modified oligonucleotides are supplied exclusively for research applications.
| Property |
Typical Value |
| Modification |
2′-Deoxyisocytidine (iso-dC) |
| Modification Type |
Artificial pyrimidine nucleobase |
| Parent Base |
Cytidine analog |
| Complementary Base |
2′-Deoxyisoguanosine (iso-dG) |
| Sugar |
2′-Deoxyribose |
| Compatible Oligos |
DNA oligonucleotides |
| Typical Applications |
Artificial base pairing, synthetic biology, DNA nanotechnology, aptamer engineering, molecular diagnostics |
Product Information
2'-Deoxyisocytidine, Iso-dC
iso-dC, 2′-Deoxyisocytidine
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Switzer C, Moroney SE, Benner SA. Enzymatic incorporation of a new base pair into DNA and RNA. Journal of the American Chemical Society. 1989;111(21):8322-8323. DOI: 10.1021/ja00203a066
- Piccirilli JA, Krauch T, Moroney SE, Benner SA. Enzymatic incorporation of a new base pair into DNA and RNA extends the genetic alphabet. Nature. 1990;343:33-37. DOI: 10.1038/343033a0
- Benner SA, Sismour AM. Synthetic biology. Nature Reviews Genetics. 2005;6(7):533-543. DOI: 10.1038/nrg1637
- Herdewijn P, ed. Modified Nucleosides in Biochemistry, Biotechnology and Medicine. Wiley-VCH; 2008.
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