Contact Us
800.227.0627

Long Trebler Branch Oligo Synthesis

Bio-Synthesis provides tree-like branching DNA or RNA synthesis by incorporating a long trebler at 5' end of an oligonucleotide using standard oligonucleotide synthesis chemistry. Dendrimers with a positively charged surfaces interact strongly with nucleic acids, helping to transport them through the membranes of cells1; dendrimers with internal cavities interact non-covalently with guest molecules to give guest-host systems2. In oligonucleotide chemistry, branched oligonucleotides4 were used for signal amplification in hybridization tests, making it possible to detect the target at levels under 100 molecules/mL3.  Fluorescent signal also increases in porportion to the number of 5' ends. When using a dendrimeric oligonucleotide as a PCR primer, the strand bearing the dendrimer is resistant to degradation by T7 Gene 6 exonuclease, making it easy to convert the double-stranded product of the PCR to a multiply labelled, single-stranded probe. Enhanced stability of DNA dendrimers makes them useful as building blocks for the 'bottom up' approach to nano-assembly. These features also apply to DNA chip technology when higher temperatures are required, to melt a secondary structure in the target.

Contact us for long trebler branch oligonucleotide synthesis.

Product Information

 

Product Name:
Long Trebler Branch Oligo Synthesis

Category:

Dendrimer; Branch Synthesis

Structure:
Bio-Synthesis Inc. Oligo Structure

Purification:
HPLC

Delivery Format:
Lyophilized

Shipping Conditions:
Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.


References/Citations:

1. G.R. Newcome, C.N. Moorefield, and F. Vogtle, Dendritic Molecules: Concepts, Synthesis, Perspectives; VCH Publishers: 1996.
2. O. Boussif, et al., Proc Natl Acad Sci U S A, 1995, 92, 7297-301.
3. J.F.G.A Jansen, et.al., Science, 1994, 266, 1226.
4. T. Horn and M.S. Urdea, Nucleic Acids Res, 1989, 17, 6959-67.
5. M.L. Collins, et al., Nucleic Acids Res, 1997, 25, 2979-84.
6. M.S. Shchepinov and E.M. Southern, Russ. J. Bioorg. Chem., 1998, 24, 794.
7. M.S. Shchepinov, I.A. Udalova, A.J. Bridgman, and E.M. Southern, Nucleic Acids Res, 1997, 25, 4447-4454.
8. C. Lehmann, Y.Z. Xu, C. Christodoulou, Z.K. Tan, and M.J. Gait, Nucleic Acids Res., 1989, 17, 2379.
9. E.M. Southern, et al., Nucleic Acids Research, 1994, 22, 1368-1373.
10. E.M. Southern, U. Maskos, and J.K. Elder, Genomics, 1992, 13, 1008-17.
11. M.S. Shchepinov, K.U. Mir, J.K. Elder, M.D. Frank-Kamenetskii, and E.M. Southern, Nucleic Acids Res, 1999, 27, 3035-41.
12. A.C. Pease, D. Solas, E.J. Sullivan, M.T. Cronin, C.P. Holmes, and S.P. Fodor, Proc Natl Acad Sci U S A, 1994, 91, 5022-6.
13. G.M. Whitesides, J.P. Mathias, and C.T. Seto, Science, 1991, 254, 1312-9.

Technical Resources

Technical & Educational
Articles
Frequently
Asked Questions
Scientific
Literature
Scientific Apps
and Calculators