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Bio-Synthesis offers Alexa 790 fluorescent dye oligonucleotide labeling services. Alexa 790 is a long wave-length dye which provides near-infrared fluorescence excitation and emission maxima of 784 and 814 nm. Alexa Fluor® 790 dye has spectral properties similar to those of indocyanine green (ICG) and IRDye 800 dyes (LI-COR Biosciences). This fluorophore is especially useful for researchers who require an amine-reactive, near-infrared label for small animal in vivo imaging (SAIVI) applications, as well as for multicolor analysis with Alexa Fluor® 680 dye and the LI-COR Odyssey infrared imaging system.
Alexa 790 dye can be incorporated at any position within an Oligonucleotide. A primary amine modified oligonucleotide is used to covalently attach with dye via NHS conjugation chemistry; other cross-linking chemistries are also available depending on particular project specifications. Dual HPLC purification is used to remove failure sequences during first HPLC purification, after labeling, the second HPLC is performed to remove unlabeled oligonucleotides and excessive dye in order to obtain a full length labeled oligonucleotide.
Contact us for Alexa 790 fluorescent dye oligonucleotide labeling services.
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-20°C To -70°C Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
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