Alexa 594, Red Emission

Alexa Fluor® 594 (AF594) is a premium red fluorescent dye developed for high-performance fluorescence imaging and detection. Its excellent brightness, superior photostability, and high fluorescence quantum yield make it one of the most widely used fluorophores for molecular biology, fluorescence microscopy, and bioanalytical applications. Compared with traditional red fluorophores, Alexa Fluor 594 provides improved resistance to photobleaching, enabling prolonged image acquisition and more reliable quantitative fluorescence measurements.

Alexa Fluor 594 is commonly conjugated to DNA, RNA, PNA, LNA, siRNA, antisense oligonucleotides (ASOs), aptamers, peptides, proteins, and other amino-modified biomolecules through NHS ester chemistry, forming a stable amide bond with primary amino groups. Alternative conjugation strategies, including click chemistry, may also be employed depending on the desired labeling approach.

The dye exhibits excitation and emission maxima of approximately 590 nm and 617 nm, respectively, making it compatible with 561 nm and 594 nm laser systems commonly used on confocal microscopes, fluorescence microscopes, imaging cytometers, and flow cytometers. Alexa Fluor 594 offers excellent spectral separation from Alexa Fluor® 488, Alexa Fluor® 555, Alexa Fluor® 647, Cy3, and FITC, making it particularly valuable for multicolor fluorescence experiments.

Alexa Fluor 594-labeled oligonucleotides are widely used for:

• Fluorescence microscopy
• Confocal microscopy
• Fluorescence in situ hybridization (FISH)
• Flow cytometry
• Live-cell imaging
• Molecular diagnostics
• qPCR probe development
• RNA and DNA imaging
• Aptamer imaging
• Multiplex fluorescence assays

Bio-Synthesis offers custom Alexa Fluor® 594-labeled oligonucleotides for DNA, RNA, PNA, LNA, siRNA, ASOs, molecular beacons, aptamers, and other synthetic nucleic acids. Alexa Fluor 594 can be incorporated at the 5′ end, 3′ end, or internal amino-modified positions, and may be combined with numerous additional modifications including quenchers, biotin, thiol modifiers, amino modifiers, PEG linkers, click chemistry handles, GalNAc, cholesterol, peptides, lipid conjugates, and other custom chemistries.

Each labeled oligonucleotide is synthesized under stringent quality standards and is available with optional HPLC purification, together with analytical characterization by HPLC, LC-MS, MALDI-TOF, UV-Vis spectroscopy, and fluorescence analysis to verify labeling efficiency, sequence identity, and product purity.

Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. Equivalent red fluorophores may be available upon request for projects requiring alternative dyes or specific licensing considerations.

 

Product Information

 

Product Name:

Alexa 594, Red Emission

Category:

Fluorescent dye Label, Texas Red

Modification Code:

[Alexa594]

Excitation (nm):

590 nm

            

Emission (nm):

617 nm

            

Extinction Coefficient, ε(λ):

73,000

Structure:

Purification:

Dual HPLC required

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

References/Citations:

1. Thermo Fisher Scientific. Alexa Fluor® 594 Dye Technical Information. Thermo Fisher Scientific
2. Panchuk-Voloshina N, Haugland RP, Bishop-Stewart J, et al. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. Journal of Histochemistry & Cytochemistry. 1999;47(9):1179-1188. DOI: 10.1177/002215549904700909
3. Lavis LD, Raines RT. Bright ideas for chemical biology. ACS Chemical Biology. 2008;3(3):142-155. DOI: 10.1021/cb700248m
4. Hermanson GT. Bioconjugate Techniques. 3rd ed. Academic Press; 2013.

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