Alexa 633, Far Red Emission

Alexa Fluor® 633 (AF633) Oligonucleotide Labeling

Alexa Fluor® 633 (AF633) is a high-performance far-red fluorescent dye designed for sensitive fluorescence imaging and analytical detection. It combines exceptional brightness, high quantum yield, and excellent photostability, making it an ideal fluorophore for advanced molecular biology, cell biology, and diagnostic applications.

Alexa Fluor® 633 is commonly conjugated to DNA, RNA, PNA, LNA, siRNA, antisense oligonucleotides (ASOs), aptamers, peptides, proteins, and other amino-modified biomolecules using NHS ester chemistry, which forms a stable amide bond with primary amino groups. The dye may also be incorporated through click chemistry or other orthogonal conjugation strategies for specialized applications.

The dye exhibits excitation and emission maxima of approximately 632 nm and 647 nm, respectively, making it highly compatible with 633 nm He-Ne lasers and many 635 nm laser systems used on confocal microscopes, flow cytometers, fluorescence microscopes, and imaging instruments. Alexa Fluor® 633 offers excellent spectral separation from green, yellow, and orange fluorophores, making it an excellent choice for multicolor fluorescence imaging and multiplex assays.

Alexa Fluor® 633-labeled oligonucleotides are widely used for:

• Confocal microscopy
• Fluorescence microscopy
• Flow cytometry
• Fluorescence in situ hybridization (FISH)
• Live-cell imaging
• Molecular diagnostics
• DNA and RNA imaging
• Aptamer imaging
• Cell labeling
• Multiplex fluorescence assays

Bio-Synthesis offers custom Alexa Fluor® 633-labeled oligonucleotides for DNA, RNA, PNA, LNA, siRNA, ASOs, molecular beacons, aptamers, and other synthetic nucleic acids. Alexa Fluor® 633 can be incorporated at the 5′ end, 3′ end, or internal amino-modified positions, and may be combined with numerous additional modifications including quenchers, biotin, thiol modifiers, amino modifiers, PEG linkers, click chemistry handles, GalNAc, cholesterol, peptides, lipid conjugates, and other custom chemistries.

Each labeled oligonucleotide is synthesized under stringent quality standards and is available with optional HPLC purification, together with analytical characterization by HPLC, LC-MS, MALDI-TOF, UV-Vis spectroscopy, and fluorescence analysis to verify labeling efficiency, sequence identity, and product purity.
 

Trademark Notice: Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. Bio-Synthesis provides custom conjugation services using customer-specified or commercially available fluorescent dyes and is not affiliated with or endorsed by Thermo Fisher Scientific.

Product Information

 

Product Name:

Alexa 633, Far Red Emission

Category:

Fluorescent dye Label, far-red dye

Modification Code:

[Alexa633]

Excitation (nm):

632

            

Emission (nm):

647

            

Extinction Coefficient, ε(λ):

159,000

Structure:

Purification:

Dual HPLC required

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

References/Citations:

1. Thermo Fisher Scientific. Alexa Fluor® 633 Fluorescent Dye Technical Information. Thermo Fisher Scientific
2. Panchuk-Voloshina N, Haugland RP, Bishop-Stewart J, et al. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. Journal of Histochemistry & Cytochemistry. 1999;47(9):1179-1188. DOI: 10.1177/002215549904700909
3. Lavis LD, Raines RT. Bright ideas for chemical biology. ACS Chemical Biology. 2008;3(3):142-155. DOI: 10.1021/cb700248m
4. Hermanson GT. Bioconjugate Techniques. 3rd ed. Academic Press; 2013.

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