Alexa 700, Near-IR Emission
Alexa Fluor® 700 (AF700) Oligonucleotide Labeling
Alexa Fluor® 700 (AF700) is a high-performance near-infrared (NIR) fluorescent dye developed for sensitive fluorescence detection and multicolor imaging applications. Operating in the far-red/near-infrared region of the spectrum, Alexa Fluor 700 minimizes interference from biological autofluorescence while providing excellent brightness, high quantum yield, and superior resistance to photobleaching.
Alexa Fluor 700 is commonly conjugated to DNA, RNA, PNA, LNA, siRNA, antisense oligonucleotides (ASOs), aptamers, peptides, proteins, and other amino-modified biomolecules through NHS ester chemistry, forming a stable amide bond with primary amino groups. Alternative conjugation approaches, including click chemistry, may also be employed depending on the labeling strategy.
The dye exhibits excitation and emission maxima of approximately 696 nm and 719 nm, respectively, making it compatible with 633 nm, 635 nm, 640 nm, and 685–695 nm laser systems commonly used in advanced flow cytometers, confocal microscopes, fluorescence imaging systems, and multiplex detection platforms. Alexa Fluor 700 offers excellent spectral separation from Alexa Fluor® 488, Alexa Fluor® 555, Alexa Fluor® 594, Alexa Fluor® 647, and other visible fluorophores, making it particularly useful for high-parameter fluorescence experiments.
Alexa Fluor 700-labeled oligonucleotides are widely used for:
- Near-infrared fluorescence imaging
- Flow cytometry
- Confocal microscopy
- Fluorescence in situ hybridization (FISH)
- Molecular diagnostics
- RNA and DNA imaging
- Aptamer imaging
- Multiplex fluorescence assays
- Cell labeling
- High-content imaging
Bio-Synthesis offers custom Alexa Fluor® 700-labeled oligonucleotides for DNA, RNA, PNA, LNA, siRNA, ASOs, molecular beacons, aptamers, and other synthetic nucleic acids. Alexa Fluor 700 can be incorporated at the 5′ end, 3′ end, or internal amino-modified positions, and may be combined with numerous additional modifications including quenchers, biotin, thiol modifiers, amino modifiers, PEG linkers, click chemistry handles, GalNAc, cholesterol, peptides, lipid conjugates, and other custom chemistries.
Each labeled oligonucleotide is synthesized under stringent quality standards and is available with optional HPLC purification, together with analytical characterization by HPLC, LC-MS, MALDI-TOF, UV-Vis spectroscopy, and fluorescence analysis to verify labeling efficiency, sequence identity, and product purity.
| Property |
Typical Value |
| Fluorophore |
Alexa Fluor® 700 (AF700) |
| Fluorescence Color |
Near-Infrared (NIR) |
| Typical Reactive Group |
NHS ester (or other conjugation formats) |
| Target Functional Group |
Primary amino groups |
| Bond Formed |
Stable amide bond |
| Excitation Maximum |
696 nm |
| Emission Maximum |
719 nm |
| Laser Compatibility |
633, 635, 640 and 685–695 nm lasers |
| Filter Compatibility |
Alexa Fluor® 700 / APC-R700 filter sets |
| Compatible Oligos |
DNA, RNA, PNA, LNA, siRNA, ASO, Aptamers |
| Typical Labeling Sites |
5′ end, 3′ end, internal amino modifiers |
Trademark Notice: Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. Bio-Synthesis provides custom conjugation services using customer-specified or commercially available fluorescent dyes and is not affiliated with or endorsed by Thermo Fisher Scientific.
Product Information
Alexa 700, Near-IR Emission
Fluorescent dye Label, Near infrared Long Wavelength Dyes
Extinction Coefficient, ε(λ):
196,000
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Thermo Fisher Scientific. Alexa Fluor® 700 Dye Technical Information. Thermo Fisher Scientific
- Panchuk-Voloshina N, Haugland RP, Bishop-Stewart J, et al. Alexa dyes, a series of new fluorescent dyes that yield exceptionally bright, photostable conjugates. Journal of Histochemistry & Cytochemistry. 1999;47(9):1179-1188. DOI: 10.1177/002215549904700909
- Lavis LD, Raines RT. Bright ideas for chemical biology. ACS Chemical Biology. 2008;3(3):142-155. DOI: 10.1021/cb700248m
- Hermanson GT. Bioconjugate Techniques. 3rd ed. Academic Press; 2013.
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