Alexa Fluor® Dye Listing
Alexa Fluor® Dye Modification Listing
Alexa Fluor® dyes are bright, photostable fluorescent labels used for DNA, RNA, PNA, peptide, protein, antibody, qPCR probe, FISH probe, and fluorescence imaging applications. This table follows the same listing format used for the Dyomics® fluorescent dye modification table, including separate excitation color and emission color columns.
| Alexa Fluor™ Dye |
Ex (nm) |
Em (nm) |
Recommended Laser |
Excitation Color |
Emission Color |
Typical Equivalent |
| Alexa Fluor™ 350 |
343 |
441 |
350 / 355 nm |
UV |
Blue |
AMCA, coumarin |
| Alexa Fluor™ 405 |
401 |
422 |
405 nm |
Violet |
Violet |
— |
| Alexa Fluor™ Plus 405 PLUS |
404 |
455 |
405 nm |
Violet |
Blue |
— |
| Alexa Fluor™ 430 |
431 |
540 |
405 / 445 nm |
Blue |
Green-Yellow |
— |
| Alexa Fluor™ 488 |
499 |
520 |
488 nm |
Blue |
Green |
Cy2, FITC (fluorescein) |
| Alexa Fluor™ Plus 488 PLUS |
493 |
518 |
488 nm |
Blue |
Green |
— |
| Alexa Fluor™ 514 |
518 |
543 |
514 nm |
Green |
Green-Yellow |
— |
| Alexa Fluor™ 532 |
534 |
553 |
532 nm |
Green |
Yellow |
— |
| Alexa Fluor™ 546 |
561 |
572 |
543 / 561 nm |
Yellow-Green |
Orange |
— |
| Alexa Fluor™ 555 |
553 |
568 |
532 / 561 nm |
Green |
Orange |
Cy3, TRITC (rhodamine) |
| Alexa Fluor™ Plus 555 PLUS |
558 |
572 |
561 nm |
Yellow-Green |
Orange |
— |
| Alexa Fluor™ 561 |
556 |
569 |
561 nm |
Yellow-Green |
Orange |
— |
| Alexa Fluor™ 568 |
579 |
603 |
561 nm |
Orange |
Orange-Red |
Rhodamine Red |
| Alexa Fluor™ 594 |
590 |
618 |
594 nm |
Orange |
Red |
Texas Red |
| Alexa Fluor™ Plus 594 PLUS |
590 |
618 |
594 nm |
Orange |
Red |
— |
| Alexa Fluor™ 633 |
631 |
650 |
633 nm |
Red |
Far Red |
— |
| Alexa Fluor™ 635 |
633 |
648 |
633 / 635 nm |
Red |
Far Red |
— |
| Alexa Fluor™ 647 |
650 |
671 |
633 / 640 nm |
Red |
Far Red |
Cy5 |
| Alexa Fluor™ Plus 647 PLUS |
658 |
675 |
633 / 640 nm |
Far Red |
Deep Red |
— |
| Alexa Fluor™ 660 |
663 |
691 |
633 / 640 nm |
Far Red |
Deep Red |
— |
| Alexa Fluor™ 680 |
681 |
704 |
670 / 680 nm |
Far Red |
Near-IR |
Cy5.5, IR680 |
| Alexa Fluor™ Plus 680 PLUS |
687 |
704 |
670 / 680 nm |
Deep Red |
Near-IR |
— |
| Alexa Fluor™ 700 |
696 |
719 |
690 / 700 nm |
Near-IR |
Near-IR |
— |
| Alexa Fluor™ 750 |
752 |
776 |
750 / 785 nm |
Near-IR |
Near-IR |
Cy7 |
| Alexa Fluor™ Plus 750 PLUS |
755 |
780 |
750 / 785 nm |
Near-IR |
Near-IR |
— |
| Alexa Fluor™ 790 |
782 |
805 |
785 nm |
Near-IR |
Near-IR |
— |
| Alexa Fluor™ Plus 800 PLUS |
789 |
794 |
785 nm |
Near-IR |
Near-IR |
— |
Technical note: Excitation and emission maxima are based on data published in the Thermo Fisher Scientific Alexa Fluor™ Brand Overview. Spectral values may vary slightly depending on solvent, pH, conjugation chemistry, biomolecule, labeling position, and measurement conditions. Recommended laser lines are provided as general instrument-selection guidance and may not correspond exactly to the excitation maximum.
Trademark and data notice: Alexa Fluor™ and Alexa Fluor™ Plus are trademarks of Thermo Fisher Scientific Inc. and/or its subsidiaries. Spectral data are based on information published by Thermo Fisher Scientific and are provided for general reference. Bio-Synthesis, Inc. is an independent custom synthesis and bioconjugation service provider and is not affiliated with, sponsored by, or endorsed by Thermo Fisher Scientific. Availability of individual dyes for custom oligonucleotide labeling is subject to reagent availability, compatible reactive-dye format, sequence design, labeling position, and technical feasibility review.
Trademark Notice: Alexa Fluor® is a registered trademark of Thermo Fisher Scientific. Bio-Synthesis provides custom oligonucleotide labeling with compatible Alexa Fluor® fluorescent dyes and is not affiliated with or endorsed by Thermo Fisher Scientific.
Product Information
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.
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