2'-F-arabinonucleic acid (2'-F-ANA) Oligonucleotides
2'-F-Arabinonucleic Acid (2'-F-ANA) Oligonucleotides
2'-F-Arabinonucleic Acid (2'-F-ANA) is a chemically modified nucleic acid in which the 2'-hydroxyl group of arabinose is replaced by a fluorine atom. Unlike conventional RNA, which contains ribose, or DNA, which contains deoxyribose, FANA incorporates an arabinose sugar possessing a fluorine atom in the 2' position. This subtle structural modification dramatically improves the biological and physicochemical properties of oligonucleotides while preserving efficient Watson-Crick base pairing.
The fluorine substitution increases the stability of the oligonucleotide against nuclease degradation and enhances hybridization with complementary RNA targets. One of the unique advantages of 2'-F-ANA is its ability to recruit RNase H, allowing selective degradation of target RNA after duplex formation. This distinguishes FANA from several other high-affinity sugar modifications that inhibit RNase H activity, making FANA particularly attractive for antisense oligonucleotide (ASO) applications.
Compared with unmodified DNA or RNA, 2'-F-ANA oligonucleotides offer numerous advantages, including:
- Increased resistance to exonucleases and endonucleases
- Enhanced affinity toward complementary RNA targets
- Excellent compatibility with RNase H-mediated RNA cleavage
- Improved serum stability
- Reduced susceptibility to enzymatic degradation
- Compatibility with gapmer and chimeric oligonucleotide designs
- Excellent performance in both in vitro and in vivo research
FANA residues can be strategically incorporated into antisense gapmers, steric-blocking oligonucleotides, siRNA analogs, aptamers, and other functional nucleic acids to optimize stability, potency, and pharmacological performance. They are frequently combined with other sugar modifications such as 2'-O-methyl (2'-OMe), 2'-Fluoro RNA (2'-F RNA), LNA, BNA, phosphorothioate (PS) backbones, and additional conjugation chemistries to achieve the desired biological profile.
Bio-Synthesis provides custom synthesis of 2'-F-ANA oligonucleotides in a wide range of sequence lengths and modification formats. FANA nucleotides can be incorporated individually or throughout an oligonucleotide and combined with fluorescent dyes, quenchers, amino modifiers, biotin, PEG linkers, click chemistry handles, GalNAc conjugates, peptides, lipids, and numerous other functional groups. Each oligonucleotide is manufactured under stringent quality standards and is available with HPLC or PAGE purification and comprehensive analytical characterization, including LC-MS or MALDI-TOF, depending on project requirements.
Because of their unique balance of high target affinity, nuclease resistance, and RNase H compatibility, 2'-F-ANA oligonucleotides have become an important platform for antisense therapeutics, functional genomics, gene silencing, aptamer engineering, and next-generation nucleic acid technologies.
Product Information
2'-F-arabinonucleic acid (2'-F-ANA) Oligonucleotides
[FANA-A[, [FANA-C], [FANA-G], [FANA-T], [FANA-U]
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
Yang X, Haecker SE, Su X, et al.
2'-F-Arabinonucleic acid modifications improve antisense oligonucleotide activity.
Nucleic Acids Research.
Crooke ST.
Antisense Drug Technology: Principles, Strategies, and Applications.
2nd Edition. CRC Press.
Damha MJ.
Fluorinated Arabinonucleic Acids for Therapeutic Oligonucleotide Development.
Various publications describing FANA chemistry, RNase H compatibility, and antisense applications.
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