ATTO 610, Red Emission

ATTO® 610 is a premium far-red fluorescent dye designed for high-performance fluorescence imaging, molecular diagnostics, and analytical applications. The dye combines exceptional brightness, excellent fluorescence quantum yield, and remarkable resistance to photobleaching, making it well suited for demanding fluorescence experiments requiring stable and sensitive signal detection.

ATTO® 610 is commonly conjugated to DNA, RNA, PNA, LNA, siRNA, antisense oligonucleotides (ASOs), aptamers, peptides, proteins, and other amino-modified biomolecules using NHS ester chemistry, forming a stable amide bond with primary amino groups. Alternative labeling approaches, including click chemistry and other orthogonal conjugation methods, may also be employed depending on the experimental design.

The dye exhibits excitation and emission maxima of approximately 615 nm and 634 nm, respectively, making it highly compatible with 594 nm, 610 nm, and 633 nm laser systems commonly found on confocal microscopes, fluorescence microscopes, imaging systems, and flow cytometers. ATTO® 610 provides excellent spectral separation from blue, green, orange, and near-infrared fluorophores, making it particularly valuable for multicolor fluorescence imaging and multiplex detection.

ATTO® 610-labeled oligonucleotides are widely used for:

• Fluorescence microscopy
• Confocal microscopy
• Fluorescence in situ hybridization (FISH)
• Flow cytometry
• Live-cell imaging
• Molecular diagnostics
• DNA and RNA imaging
• Aptamer imaging
• Cell labeling
• Multiplex fluorescence assays

Bio-Synthesis offers custom ATTO® 610-labeled oligonucleotides for DNA, RNA, PNA, LNA, siRNA, ASOs, molecular beacons, aptamers, and other synthetic nucleic acids. ATTO® 610 can be incorporated at the 5′ end, 3′ end, or internal amino-modified positions, and may be combined with numerous additional modifications including quenchers, biotin, thiol modifiers, amino modifiers, PEG linkers, click chemistry handles, GalNAc, cholesterol, peptide conjugates, lipid conjugates, and other custom chemistries.

Each labeled oligonucleotide is synthesized under stringent quality standards and is available with optional HPLC purification, together with analytical characterization by HPLC, LC-MS, MALDI-TOF, UV-Vis spectroscopy, and fluorescence analysis to verify labeling efficiency, sequence identity, and product purity.

Trademark Notice: ATTO® is a registered trademark of ATTO-TEC GmbH. Bio-Synthesis provides custom conjugation services using customer-specified or commercially available fluorescent dyes and is not affiliated with or endorsed by ATTO-TEC GmbH.

Product Information

 

Product Name:

ATTO 610, Red Emission

Category:

Fluorescent dye Label, Alexa Fluor 610

Modification Code:

[ATTO610]

Chemical Formula:

C25H30N2O

Exact Mass:

374.24

Formula Weight:

374.53

Excitation (nm):

615 nm

Emission (nm):

634 nm

Extinction Coefficient, ε(λ):

150,000

Structure:

Purification:

dual HPLC/SEC

Delivery Format:

Lyophilized

Shipping Conditions:

Room Temperature

Storage Conditions:

-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.

References/Citations:

1. ATTO-TEC GmbH. ATTO® 610 Fluorescent Label. https://www.atto-tec.com/
2. ATTO-TEC GmbH. ATTO Dyes Product Guide. Comprehensive fluorescence spectra, photophysical properties, and conjugation chemistries.
3. Lavis LD, Raines RT. Bright ideas for chemical biology. ACS Chemical Biology. 2008;3(3):142-155. DOI: 10.1021/cb700248m
4. Hermanson GT. Bioconjugate Techniques. 3rd ed. Academic Press; 2013.

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