Azido PEG12
Azido PEG12 is a versatile click chemistry modification that combines a terminal azide functional group (–N₃) with a flexible polyethylene glycol (PEG12) spacer. The twelve ethylene glycol units create a hydrophilic linker approximately 5–6 nm in extended length, improving molecular flexibility, increasing water solubility, and minimizing steric interference between the oligonucleotide and its conjugated payload.
The terminal azide participates in highly selective bioorthogonal click chemistry, allowing efficient conjugation with terminal alkynes through copper-catalyzed azide-alkyne cycloaddition (CuAAC) or with strained cyclooctynes (DBCO, BCN, ADIBO, DIBAC) using strain-promoted azide-alkyne cycloaddition (SPAAC). These reactions produce stable triazole linkages with excellent efficiency and minimal side reactions.
Compared with shorter PEG linkers such as PEG3, PEG6, or PEG9, PEG12 provides greater separation between the oligonucleotide and the attached molecule. This additional spacing can improve target accessibility, reduce steric hindrance, enhance hybridization performance after conjugation, and improve the behavior of larger biomolecular conjugates.
Bio-Synthesis offers custom Azido PEG12-modified oligonucleotides for DNA, RNA, PNA, LNA, siRNA, antisense oligonucleotides (ASOs), aptamers, molecular beacons, and other synthetic nucleic acids. The modification may be introduced at the 5′ end, 3′ end, or selected internal positions and is compatible with numerous additional modifications including fluorophores, quenchers, biotin, thiol modifiers, amino modifiers, GalNAc, cholesterol, lipids, peptides, proteins, antibodies, nanoparticles, and other custom conjugates.
Typical applications include:
- CuAAC click chemistry
- Copper-free SPAAC conjugation
- Antibody-oligonucleotide conjugates
- Protein-oligonucleotide conjugates
- Peptide conjugation
- Fluorescent dye labeling
- Nanoparticle functionalization
- DNA nanotechnology
- Aptamer conjugation
- Therapeutic oligonucleotide research
Each modified oligonucleotide is synthesized using high-quality solid-phase phosphoramidite chemistry and is available with optional HPLC or PAGE purification. Comprehensive analytical characterization includes HPLC, LC-MS, MALDI-TOF, UV spectroscopy, and additional quality control testing to verify sequence identity, purity, and successful incorporation of the Azido PEG12 modification.
| Property |
Typical Value |
| Modification |
Azido PEG12 |
| Functional Group |
Terminal azide (–N₃) |
| Spacer |
PEG12 (12 ethylene glycol units) |
| Typical Length |
~5–6 nm (fully extended) |
| Conjugation Chemistry |
CuAAC and SPAAC click chemistry |
| Compatible Reaction Partners |
Terminal alkynes, DBCO, BCN, DIBAC, ADIBO |
| Typical Attachment |
5′ end, 3′ end, or internal modification |
| Compatible Oligos |
DNA, RNA, PNA, LNA, siRNA, ASO, Aptamers |
| Typical Applications |
Click chemistry, PEGylation, antibody conjugation, protein conjugation, fluorescent labeling, nanotechnology |
Product Information
Click Chemistry & Bioorthogonal Modifications
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.
References/Citations:
-
Kolb HC, Finn MG, Sharpless KB.
Click Chemistry: Diverse Chemical Function from a Few Good Reactions.
Angewandte Chemie International Edition. 2001;40(11):2004-2021.
DOI
-
Rostovtsev VV, Green LG, Fokin VV, Sharpless KB.
A Stepwise Huisgen Cycloaddition Process: Copper(I)-Catalyzed Azide-Alkyne Cycloaddition.
Angewandte Chemie International Edition. 2002;41(14):2596-2599.
-
Jewett JC, Bertozzi CR.
Cu-free click cycloaddition reactions in chemical biology.
Chemical Society Reviews. 2010;39:1272-1279.
DOI: 10.1039/B901970G
-
Hermanson GT.
Bioconjugate Techniques.
3rd ed. Academic Press; 2013.
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