BODIPY 650/665 Fluor Dye, Far Red Emission
BODIPY™ 650/665 is a far-red fluorescent member of the BODIPY fluorophore family, recognized for its exceptional brightness, excellent photostability, high quantum yield, and narrow emission bandwidth. Compared with conventional rhodamine or cyanine dyes, BODIPY fluorophores often provide improved spectral resolution, making them well suited for multiplex fluorescence experiments.
The dye is optimized for excitation with 633–640 nm red lasers and emits bright far-red fluorescence around 665 nm. These optical properties make BODIPY™ 650/665 particularly useful for fluorescence microscopy, flow cytometry, confocal imaging, fluorescence polarization, FRET assays, biosensor development, and live-cell imaging.
Bio-Synthesis offers custom BODIPY™ 650/665-labeled oligonucleotides for DNA, RNA, PNA, LNA/BNA, aptamers, siRNA, antisense oligonucleotides, CRISPR guide RNAs, molecular beacons, and diagnostic probes. Depending on the synthesis strategy, the fluorophore may be incorporated at the 5′ terminus, 3′ terminus, or internal positions.
Typical applications include:
- Fluorescence microscopy
- Confocal microscopy
- Flow cytometry
- Live-cell imaging
- Molecular imaging
- FRET acceptor labeling
- Hybridization probes
- qPCR probe development
- Biosensors
- DNA nanotechnology
- Multiplex fluorescence assays
Typical Specifications
| Property |
Specification |
| Modification |
BODIPY™ 650/665 |
| Modification Type |
Fluorescent dye |
| Synthesis Chemistry |
Phosphoramidite or NHS ester (depending on the labeling strategy) |
| Dye Family |
BODIPY (boron-dipyrromethene) |
| Spacer |
C3 or C6 linker (depending on reagent) |
| Excitation Maximum |
Approximately 650 nm |
| Excitation Color |
Red |
| Emission Maximum |
Approximately 665 nm |
| Emission Color |
Far Red |
| Recommended Laser |
633 nm or 640 nm |
| Typical Placement |
5′ end, 3′ end, or internal positions |
| Compatible Oligonucleotides |
DNA, RNA, PNA, LNA/BNA, siRNA, ASOs, aptamers, molecular probes |
| Typical Applications |
Flow cytometry, fluorescence microscopy, FRET, hybridization probes, molecular imaging, biosensors, multiplex fluorescence assays |
| Comparable Dyes |
Alexa Fluor® 647, ATTO® 647N, DY-647, CF® 647, Cy5® (approximate spectral equivalents) |
Product Information
BODIPY 650/665 Fluor Dye, Far Red Emission
Extinction Coefficient, ε(λ):
102,000
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Loudet A, Burgess K. BODIPY dyes and their derivatives: syntheses and spectroscopic properties. Chemical Reviews. 2007;107(11):4891-4932.
- Ulrich G, Ziessel R, Harriman A. The chemistry of fluorescent BODIPY dyes. Angewandte Chemie International Edition. 2008;47:1184-1201.
- Lakowicz JR. Principles of Fluorescence Spectroscopy. 3rd Edition. Springer; 2006.
- Hermanson GT. Bioconjugate Techniques. 3rd Edition. Academic Press; 2013.
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