Cascade Yellow Fluor Dye, Yellow Emission
Cascade Yellow™ is a fluorescent dye developed for applications requiring efficient excitation with violet or blue light and bright yellow fluorescence emission. The fluorophore exhibits a relatively large Stokes shift, helping minimize excitation light bleed-through while improving detection sensitivity in fluorescence-based assays.
When conjugated to DNA, RNA, PNA, aptamers, or other synthetic oligonucleotides, Cascade Yellow™ provides a stable fluorescent label suitable for molecular biology, diagnostics, and imaging applications. The dye is frequently used in fluorescence microscopy, flow cytometry, hybridization assays, biosensor development, and multiplex fluorescence experiments where separation from blue-, green-, or red-emitting fluorophores is desirable.
Bio-Synthesis offers custom Cascade Yellow™-modified oligonucleotides for DNA, RNA, PNA, LNA/BNA, aptamers, siRNA, antisense oligonucleotides, CRISPR guide RNAs, molecular beacons, and diagnostic probes. Depending on the synthesis strategy, the dye can be incorporated at the 5′ terminus, 3′ terminus, or internal positions.
Typical applications include:
- Fluorescence microscopy
- Confocal microscopy
- Flow cytometry
- Hybridization probes
- Molecular beacons
- FRET acceptor applications
- Biosensor development
- DNA nanotechnology
- Multiplex fluorescence imaging
- Molecular diagnostics
Typical Specifications
| Property |
Specification |
| Modification |
Cascade Yellow™ |
| Modification Type |
Fluorescent dye |
| Synthesis Chemistry |
Phosphoramidite (direct synthesis) or NHS ester (post-synthetic conjugation), depending on the reagent used |
| Dye Family |
Cascade™ fluorophore family |
| Spacer |
C3, C6, or linker-dependent spacer (product dependent) |
| Excitation Maximum |
Approximately 400 nm |
| Excitation Color |
Violet |
| Emission Maximum |
Approximately 550 nm |
| Emission Color |
Yellow |
| Recommended Laser |
405 nm |
| Typical Placement |
5′ end, 3′ end, or internal positions |
| Compatible Oligonucleotides |
DNA, RNA, PNA, LNA/BNA, siRNA, ASOs, aptamers, molecular probes |
| Typical Applications |
Fluorescence microscopy, flow cytometry, FRET, hybridization assays, biosensors, multiplex fluorescence imaging |
| Comparable Dyes |
Alexa Fluor® 430, ATTO® 425, DY-430 (approximate spectral equivalents) |
Product Information
Cascade Yellow Fluor Dye, Yellow Emission
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Haugland RP. The Handbook: A Guide to Fluorescent Probes and Labeling Technologies. Molecular Probes (Thermo Fisher Scientific).
- Lakowicz JR. Principles of Fluorescence Spectroscopy. 3rd Edition. Springer; 2006.
- Hermanson GT. Bioconjugate Techniques. 3rd Edition. Academic Press; 2013.
- Lavis LD, Raines RT. Bright ideas for chemical biology. ACS Chemical Biology. 2008;3(3):142-155.
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