Coumarins represent small molecular weight blue fluorescent dyes that are UV-excitable. Coumarin dyes are water soluble and permeable across the cell membrane though the permeability may be reduced with the increase in charged moieties. They can be used to provide ideal contrast in multicolor context. As their fluorescence is not as bright, it may be applicable to situations wherein the targets are abundant or for cell tracking purposes to avoid being obscured by autofluorescence.
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-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at -20°C) in a nuclease-free environment should be stable for years.
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