DBCO NHS Modifier
DBCO NHS ester is a widely used bioorthogonal labeling reagent that reacts selectively with primary amines to install a dibenzocyclooctyne (DBCO) group onto oligonucleotides, peptides, proteins, antibodies, nanoparticles, and other biomolecules. In oligonucleotide synthesis, DBCO NHS ester is commonly used to modify amino-functionalized DNA, RNA, PNA, aptamers, antisense oligonucleotides, siRNA, and probes following synthesis.
The DBCO group participates in strain-promoted azide-alkyne cycloaddition (SPAAC), a copper-free click chemistry reaction that proceeds rapidly under mild aqueous conditions. Unlike CuAAC, SPAAC does not require copper catalysts, making it well suited for applications involving sensitive biomolecules, living cells, and biological systems where copper toxicity or nucleic acid damage is undesirable.
DBCO-modified oligonucleotides readily conjugate with azide-modified fluorophores, peptides, proteins, antibodies, lipids, polymers, PEG molecules, nanoparticles, carbohydrates, and surfaces, providing a versatile platform for custom bioconjugation and targeted delivery.
Typical applications include:
- Copper-free click chemistry (SPAAC)
- Antibody–oligonucleotide conjugates
- Peptide–oligonucleotide conjugates
- Nanoparticle functionalization
- Surface immobilization
- Aptamer conjugation
- Fluorescent labeling
- Targeted delivery research
- Biosensor development
- DNA nanotechnology
- Molecular imaging
Bio-Synthesis offers custom DBCO-modified oligonucleotides prepared through post-synthetic conjugation of amino-modified oligonucleotides with DBCO NHS ester, providing high-quality click-ready constructs for research applications.
Technical note: Because DBCO NHS ester is a labeling reagent rather than a phosphoramidite, the usual workflow is to synthesize an amino-modified oligonucleotide first (e.g., Amino C6 or Amino-TEG) and then react it with DBCO NHS ester to generate the final DBCO-modified oligonucleotide. This distinction is worth mentioning on your product page to avoid confusion with directly incorporable synthesis modifiers
Typical Specifications
| Property |
Specification |
| Modification |
DBCO (Dibenzocyclooctyne) |
| Reagent |
DBCO NHS Ester |
| Reactive Group |
Strained cyclooctyne |
| Conjugation Chemistry |
SPAAC (Strain-Promoted Azide-Alkyne Cycloaddition) |
| Copper Requirement |
None (Copper-free) |
| Reaction Partner |
Azide-modified molecules |
| Typical Placement |
5′ end, 3′ end, or internal amino-modified positions |
| Compatible Oligonucleotides |
DNA, RNA, PNA, LNA/BNA, siRNA, ASOs, aptamers, probes |
| Typical Applications |
Copper-free click chemistry, antibody conjugation, peptide conjugation, nanoparticle labeling, fluorescent labeling, biosensors, targeted delivery |
Product Information
copper-free click chemistry
-20°C To -70°C
Oligonucleotides are stable in solution at 4°C for up to 2 weeks. Properly reconstituted material stored at -20°C should be stable for at least 6 months. Dried DNA (when kept at 20°C) in a nuclease-free environment should be stable for years.
References/Citations:
- Agard NJ, Prescher JA, Bertozzi CR. A strain-promoted [3+2] azide-alkyne cycloaddition for covalent modification of biomolecules in living systems. Journal of the American Chemical Society. 2004;126(46):15046-15047.
- Jewett JC, Bertozzi CR. Cu-free click cycloaddition reactions in chemical biology. Chemical Society Reviews. 2010;39:1272-1279.
- Sletten EM, Bertozzi CR. Bioorthogonal chemistry: fishing for selectivity in a sea of functionality. Angewandte Chemie International Edition. 2009;48:6974-6998.
- Hermanson GT. Bioconjugate Techniques. 3rd Edition. Academic Press; 2013.
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